An infectious full-length cDNA clone of potato virus YNTN-NW, a recently reported strain of PVY that causes potato tuber necrotic ringspot disease
An infectious full-length cDNA clone of potato virus YNTN-NW, a recently reported strain of PVY that causes potato tuber necrotic ringspot disease
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DOI：
10.1007/s00705-011-1062-4
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发表时间：
2011-11-01
影响因子：
2.7
通讯作者：
Natsuaki, T.
中科院分区：
文献类型：
--
作者：
Ali, M. Chikh;Omar, A. Said;Natsuaki, T.
以引起马铃薯块茎坏死环斑病（PTNRD）的马铃薯Y病毒（PVYNTN-NW）分离物SYR-II-2-8为材料，构建了PVY感染性全长cDNA克隆。引入沉默点突变以修饰与SYR-II-2-8的P3编码区5'末端检测到的原核启动子元件相似的序列。沿着改变E. coli中，成功构建了一个稳定的全长感染性cDNA克隆，命名为p2- 8 C3。p2- 8 C3的生物学特性与SYR-II-2-8的生物学特性相同，两者均诱导马铃薯PTNRD，烟草脉坏死，并且在接种的马铃薯品种中具有相似的症状。
A full-length infectious cDNA clone of potato virus Y (PVY) was constructed based on an isolate of the PVYNTN-NW strain, SYR-II-2-8, which is able to cause potato tuber necrotic ringspot disease (PTNRD). Silent point mutations were introduced to modify sequences similar to the prokaryotic promoter elements detected at the 5' terminus of the P3 coding region of SYR-II-2-8. This, along with modification of the growing conditions of E. coli, led to the successful construction of a stable full-length infectious cDNA clone, named p2-8C3. The biological properties of p2-8C3 were identical to those of SYR-II-2-8, both of which induced PTNRD in potato, veinal necrosis in tobacco, and similar symptoms in the potato cultivars inoculated.