Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae
Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae
复制标题
DOI：
10.1016/s0378-1119(97)00031-0
复制
发表时间：
1997-06-19
期刊：
影响因子：
3.5
通讯作者：
Ohsumi, Y
中科院分区：
文献类型：
--
作者：
Funakoshi, T;Matsuura, A;Ohsumi, Y
我们在酿酒酵母中分离出了 14 个自噬缺陷的 apg 突变体（Tsukada 和 Ohsumi，1993）。其中，APG1编码一种新型Ser/Thr蛋白激酶，其激酶活性对于自噬至关重要。在寻找与APG1基因相互作用的基因的过程中，我们发现GAL1启动子控制下APG1的过度表达抑制了apg13-1突变体的自噬缺陷表型。克隆和测序分析表明APG13基因编码一种由738个氨基酸残基组成的新型亲水蛋白。 APG13 基因是组成型表达的，不是饥饿诱导型的。虽然 APG13 对于细胞增殖来说是可有可无的，但它对于在饥饿条件下维持细胞活力非常重要。 apg13 破坏者与 apg13-1 突变体一样在自噬方面存在缺陷。形态学和生化研究表明，APG1 过表达也抑制了 Delta apg13 的自噬缺陷。这些结果暗示 APG1 和 APG13 之间存在遗传相互作用。 (C) 1997 Elsevier Science B.V.
We have isolated 14 apg mutants defective in autophagy in yeast Saccharomyces cerevisiae (Tsukada and Ohsumi, 1993). Among them, APG1 encodes a novel Ser/Thr protein kinase whose kinase activity is essential for autophagy. In the course of searching for genes that genetically interact with APG1, we found that overexpression of APG1 under control of the GAL1 promoter suppressed the autophagy-defective phenotype of apg13-1 mutant. Cloning and sequencing analysis showed that the APG13 gene encodes a novel hydrophilic protein of 738 amino acid residues. APG13 gene is constitutively expressed bot not starvation-inducible. Though dispensable for cell proliferation, APG13 is important for maintenance of cell viability under starvation conditions. apg13 disruptants were defective in autophagy like apg13-1 mutants. Morphological and biochemical investigation showed that a defect in autophagy of Delta apg13 was also suppressed by APG1 overexpression. These results imply genetic interaction between APG1 and APG13. (C) 1997 Elsevier Science B.V.