Post-transcriptional regulation of tumour necrosis factor alpha production
Post-transcriptional regulation of tumour necrosis factor alpha production
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DOI：
10.1136/ard.59.suppl_1.i3
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发表时间：
2000-11-01
影响因子：
27.4
通讯作者：
Anderson, Paul
中科院分区：
文献类型：
--
作者：
Anderson, Paul
肿瘤坏死因子α（TNFα）是由活化的巨噬细胞、淋巴细胞和其他细胞产生的促炎细胞因子。1-3 TNFα的产生受转录和转录后控制。4-10 TNFα表达的转录后控制通过调节翻译起始、mRNA稳定性和多聚腺苷酸化来实现。6 7 10 TNFα转录物3 '非翻译区（3' UTR）中的腺嘌呤和尿苷（Au）富集元件（ARE）11 -13是转录后控制的重要决定因素。将该元件转移到异源报告转录物改变了报告蛋白的表达。最近已经鉴定了与ARE结合并参与转录后控制的反式调节因子。在给定细胞中表达的ARE结合蛋白被认为决定蛋白表达水平。
Tumour necrosis factor α (TNFα) is a proinflammatory cytokine produced by activated macrophages, lymphocytes and other cells. 1–3 The production of TNFα is under transcriptional and post-transcriptional control. 4–10 Post-transcriptional control of TNFα expression is achieved by regulating translational initiation, mRNA stability, and polyadenylation. 6 7 10 An adenine and uridine (AU)-rich element (ARE) in the 3’untranslated region (3’UTR) of TNFα transcripts11–13 is an important determinant of posttranscriptional control. Transfer of this element to heterologous reporter transcripts changes the expression of the reporter protein. Transacting factors that bind to the ARE and participate in post-transcriptional control have recently been identified. The ARE binding proteins expressed in a given cell are thought to determine the level of protein expression.