Sympatry of distinct mitochondrial DNA lineages in a copepod inhabiting estuarine creeks in the southeastern USA
Sympatry of distinct mitochondrial DNA lineages in a copepod inhabiting estuarine creeks in the southeastern USA
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栖息在美国东南部河口小溪的桡足类动物不同线粒体 DNA 谱系的相似性
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
J. Quattro
中科院分区：
文献类型：
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作者：
N. Schizas;B. Coull;G. Chandler;J. Quattro
抽象的。通过对线粒体细胞色素 b 基因的 348 bp 片段进行地理调查，研究了小型底栖类桡足类桡足类 Microarthridionlittorale (Poppe) 的种群遗传结构。 1997 年 1 月至 1998 年 11 月，从美国北卡罗来纳州到佐治亚州海岸的 10 个地点采集了桡足类动物。198 个个体之间的序列差异高达 4.3%，并发现了 3 个不同的线粒体进化枝，差异有 6 到 9 个核苷酸变化。我们开发了一种快速检测方法来区分线粒体进化枝，并对另外 333 个样本进行了调查。这三个谱系在十个采样点中的七个同时出现。对通过 DNA 测序测定的个体以及包括通过限制性核酸内切酶消化分型的个体的组合数据集分别进行了数据分析。分子方差分析表明，尽管地理距离和遗传距离之间没有检测到显着相关性，但总遗传方差的很大一部分可以在人群之间分配。
Abstract. The population genetic structure of the meiobenthic harpacticoid copepod Microarthridionlittorale (Poppe) was examined with a geographic survey of a 348 bp fragment of the mitochondrial cytochrome b gene. Copepods were collected from ten locations on the coast from North Carolina to Georgia, USA, from January 1997 to November 1998. Sequence divergence among 198 individuals was as much as 4.3%, and three divergent mitochondrial clades were uncovered that differed by six to nine nucleotide changes. A rapid assay was developed to distinguish among mitochondrial clades, and an additional 333 specimens were surveyed. The three lineages co-occurred in seven of ten sampling locations. Data analyses were carried out separately for individuals assayed by DNA sequencing as well as for a combined data set that included individuals typed by restriction endonuclease digestion. An analysis of molecular variance indicated that a significant proportion of the total genetic variance could be partitioned among populations, although no significant correlation between geographical and genetic distance was detected.
影响因子：
3.3
作者：
Alan R. Templeton;K. Crandall;C. Sing
通讯作者：
Alan R. Templeton;K. Crandall;C. Sing