Aggregation-based determination of mercury(II) using DNA-modified single gold nanoparticle, T-Hg(II)-T interaction, and single-particle ICP-MS
Aggregation-based determination of mercury(II) using DNA-modified single gold nanoparticle, T-Hg(II)-T interaction, and single-particle ICP-MS
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DOI：
10.1007/s00604-019-4057-6
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发表时间：
2020-01-01
影响因子：
5.7
通讯作者：
Zhao, Julia Xiaojun
中科院分区：
文献类型：
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作者：
Xing, Yuqian;Han, Juan;Zhao, Julia Xiaojun
介绍了一种基于单粒子电感耦合等离子体质谱（spICP-MS）的超灵敏测定方法。在Hg 2+（aq）存在下，由于形成众所周知的胸腺嘧啶（T）-Hg 2 +-T复合物，用单链DNA片段修饰的金纳米颗粒聚集。使用单粒子（sp）ICP-MS通过检测到的AuNP或NP聚集体的数量的总体减少来量化聚集程度。与大多数其他使用相同原理的聚集-分散与DNA修饰的金纳米颗粒的汞测定相比，这种方法具有更低的检测限（0.031 ng L-1，155 fM）和更宽的（10，000倍）线性范围（高达1 μ g L-1）。由于该方法对水样基质的干扰最小，因此也显示出良好的实用潜力。
An ultrasensitive assay is described for the detection and determination of Hg2+(aq) in water samples based on single-particle inductively-coupled plasma/mass spectrometry (spICP-MS). In the presence of Hg2+(aq), AuNPs modified with a segment of single-stranded DNA aggregate due to the formation of the well-known thymine (T)-Hg2+-T complex. Single particle (sp) ICP-MS is used quantify the degree of aggregation by the overall decrease in number of detected AuNPs or NP aggregates. Compared with most other Hg2+ assays that use the same principle of aggregation-dispersion with DNA modified AuNPs, this method has a much lower detection limit of (0.031 ng L-1, 155 fM) and a wider (10,000-fold) linear range (up to 1 mu g L-1). The method also showed good practical potential because of its minimal interference from the water sample matrix.