Diversity of Babesia bovis merozoite surface antigen genes in the Philippines.
Diversity of Babesia bovis merozoite surface antigen genes in the Philippines.
复制标题
菲律宾牛巴贝斯虫裂殖子表面抗原基因的多样性。
DOI：
10.1016/j.parint.2013.09.003
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Yokoyama N.
中科院分区：
文献类型：
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作者：
Tattiyapong M;Sivakumar T;Ybanez AP;Ybanez RH;Perez ZO;Guswanto A;Igarashi I;Yokoyama N.
牛巴贝斯虫是牛致命性巴贝斯虫病的病原体。本研究对我国北方地区的黑木耳进行了遗传多样性研究。bovisamong菲律宾牛，基于编码裂殖子表面抗原（MSA）的基因。41B用牛的阳性血液DNA样品扩增它们的msa-1、msa-2b和msa-2c基因。在系统发育分析中，themsa-1，msa-2b和msa-2c基因序列来自菲律宾B。牛阳性DNA样品分别在6个、3个和4个不同的分支中发现。所有的themsa-1序列和大多数themsa-2b序列都被发现在仅由菲律宾序列形成的分支中。虽然所有来自菲律宾的themsa-1序列与澳大利亚的msa-1序列相似，但themsa-2b序列与澳大利亚或墨西哥的msa-2b序列相似。与此相反，来自菲律宾的MSA-2C序列分布在所有的分支，虽然一个分支是专门由菲律宾的MSA-2C序列。与来自菲律宾的MSA-1、MSA-2b和MSA-2c的氨基酸序列相似性分别为62.2-100、73.1-100和67.3- 100%。本研究结果表明，B.在菲律宾，牛科动物的遗传多样性。这些信息将为今后设计和实施针对菲律宾牛巴贝斯虫病的改进免疫预防方法提供良好的基础。这项研究还产生了一组数据，这将有助于进一步了解这种重要寄生虫的全球遗传多样性。
Babesia bovis is the causative agent of fatal babesiosis in cattle. In the present study, we investigated the genetic diversity ofB. bovisamong Philippine cattle, based on the genes that encode merozoite surface antigens (MSAs). Forty-oneB. bovis-positive blood DNA samples from cattle were used to amplify themsa-1,msa-2b, andmsa-2cgenes. In phylogenetic analyses, themsa-1,msa-2b, andmsa-2cgene sequences generated from PhilippineB. bovis-positive DNA samples were found in six, three, and four different clades, respectively. All of themsa-1and most of themsa-2bsequences were found in clades that were formed only by Philippinemsasequences in the respective phylograms. While all themsa-1sequences from the Philippines showed similarity to those formed by Australianmsa-1sequences, themsa-2bsequences showed similarity to either Australian or Mexicanmsa-2bsequences. In contrast,msa-2csequences from the Philippines were distributed across all the clades of the phylogram, although one clade was formed exclusively by Philippinemsa-2csequences. Similarities among the deduced amino acid sequences of MSA-1, MSA-2b, and MSA-2c from the Philippines were 62.2–100, 73.1–100, and 67.3–100%, respectively. The present findings demonstrate thatB. bovispopulations are genetically diverse in the Philippines. This information will provide a good foundation for the future design and implementation of improved immunological preventive methodologies against bovine babesiosis in the Philippines. The study has also generated a set of data that will be useful for futher understanding of the global genetic diversity of this important parasite.