DNA mutation detection and analysis using miniaturized microfluidic systems
DNA mutation detection and analysis using miniaturized microfluidic systems
复制标题
DOI：
10.1586/14737159.6.1.29
复制
发表时间：
2006-01
影响因子：
5.1
通讯作者：
Maria Handal;V. Ugaz
中科院分区：
文献类型：
--
作者：
Maria Handal;V. Ugaz
识别发生在人群范围内的遗传序列变异是解开复杂相互作用的关键，这些相互作用是许多医学障碍和疾病的根本原因。然而，迫切需要先进的技术，以使DNA突变分析能够以比目前可达到的显著更高的通量和显著更低的成本进行。微流体系统提供了一个有吸引力的平台，以解决这些需求，通过结合的能力，执行快速分析与简化的设备格式，可以廉价地大规模生产。本文将回顾开发这些下一代系统的最新进展，并讨论与适应这些技术用于常规实验室使用相关的挑战。
Identification of genetic sequence variations occurring on a population-wide scale is key to unraveling the complex interactions that are the underlying cause of many medical disorders and diseases. A critical need exists, however, for advanced technology to enable DNA mutation analysis to be performed with significantly higher throughput and at significantly lower cost than is currently attainable. Microfluidic systems offer an attractive platform to address these needs by combining the ability to perform rapid analysis with a simplified device format that can be inexpensively mass-produced. This paper will review recent progress toward developing these next-generation systems and discuss challenges associated with adapting these technologies for routine laboratory use.