FETAL WOUND-HEALING - THE ONTOGENY OF SCAR FORMATION IN THE NONHUMAN PRIMATE
FETAL WOUND-HEALING - THE ONTOGENY OF SCAR FORMATION IN THE NONHUMAN PRIMATE
复制标题
DOI：
10.1097/00000658-199304000-00011
复制
发表时间：
1993-04-01
影响因子：
9
通讯作者：
ADZICK, NS
中科院分区：
文献类型：
--
作者：
LORENZ, HP;WHITBY, DJ;ADZICK, NS
Objective本研究确定了瘢痕形成如何在非人灵长类动物胎儿皮肤repair.Summary背景数据模型中发展，从愈合无瘢痕愈合瘢痕形成的特点在大鼠和绵羊胎儿皮肤修复的过渡。新的知识的监管过程中发生在胎儿伤口的瘢痕形成的初始阶段，可能会提供洞察瘢痕formation.Methods的早期机制，全层伤口在胎儿恒河猴唇从75至114天妊娠（n = 6，任期= 165天）。伤口收获在14天伤后和处理的组织学（苏木精和伊红，Masson的三色），以及免疫组化（人I型或III型胶原）。结果伤口愈合，完全恢复正常的组织结构在75天的妊娠胎儿。然而，在妊娠85-100天的胎仔中，伤口愈合，毛囊和皮脂腺缺失，但真皮胶原蛋白模式仍为网状，与未受伤真皮相似。在107天，一个薄的疤痕是存在于伤口，从而证明了过渡到疤痕形成之间的100和107天妊娠（早期第三个三个月）在非人类灵长类动物。结论在非人类灵长类动物胎儿，从无疤痕修复到成人型修复疤痕形成的过渡发生在早期第三个三个月。这些数据提供了对过渡过程的深入了解：瘢痕形成的个体发生最初的特征是伤口愈合，不存在表皮附属物，但具有正常的网状真皮胶原蛋白模式，我们称之为“过渡伤口”。''
Objective This study determined how scar formation develops in a non-human primate model of fetal skin repair.Summary Background Data A transition from healing scarlessly to healing with scar formation characterizes skin repair in rat and sheep fetuses. New knowledge of the regulatory processes occurring in the fetal wound at the initial stages of scar formation may provide insights into the early mechanisms of scar formation.Methods Full-thickness wounds were made in fetal rhesus monkey lips from 75 through 114 days gestation (n = 6, term = 165 days). Wounds were harvested at 14 days postwounding and processed for histology (hematoxylin & eosin, Masson's trichrome) as well as immunohistochemistry (human type I or type III collagen).Results Wounds healed with complete restoration of normal tissue architecture in the 75-day gestation fetus. However in the 85-100-day gestation fetuses, wounds healed with an absence of hair follicles and sebaceous glands, but the dermal collagen pattern remained reticular and similar to that in unwounded dermis. At 107 days, a thin scar was present in the wound, thereby demonstrating a transition to scar formation between 100 and 107 days gestation (early 3rd trimester) in the non-human primate.Conclusions In the non-human primate fetus, a transition from scarless repair to adult-type repair with scar formation occurs in the early third trimester. These data provide insight into the transition process: the ontogeny of scar formation is characterized initially by wounds healing without the presence of epidermal appendages but with a normal reticular dermal collagen pattern, which we term the ''transition wound.''