DNA methylation profiles provide a viable index for porcine pluripotent stem cells.
DNA methylation profiles provide a viable index for porcine pluripotent stem cells.
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DNA甲基化谱为猪多能干细胞提供了一个可行的指数。
DOI：
10.1002/dvg.22423
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发表时间：
2013-11
期刊：
影响因子：
1.5
通讯作者：
Nagashima, Hiroshi
中科院分区：
文献类型：
--
作者：
Arai, Yoshikazu;Ohgane, Jun;Fujishiro, Shuh-hei;Nakano, Kazuaki;Matsunari, Hitomi;Watanabe, Masahito;Umeyama, Kazuhiro;Azuma, Dai;Uchida, Naomi;Sakamoto, Nozomu;Makino, Tomohiro;Yagi, Shintaro;Shiota, Kunio;Hanazono, Yutaka;Nagashima, Hiroshi
猪诱导多能干细胞（iPSC）为转基因研究提供了有用的信息。iPSC的质量可以通过它们在嵌合体形成后分化成各种细胞类型的能力来评估。然而，猪中嵌合体形成的分析是一个劳动密集型和昂贵的过程，需要一种简单的猪iPSC评估方法。我们先前的研究鉴定了小鼠胚胎干细胞（ESC）特异性低甲基化位点（EShypo-T-DMR），并且在本研究中，从这些基因中选择36个基因用于评估猪iPSC系。基于这36个基因的甲基化谱，在5个猪iPSC中，发现iPSC系Porco罗索-4最接近小鼠多能干细胞。此外，Porco罗索-4比iPSC系更有效地促进胚泡的内细胞团（ICM），所述iPSC系显示36个基因的最低重编程（Porco罗索-622-14），表明DNA甲基化谱与ICM贡献的效率相关。此外，已知提高iPSC质量的因素（含PD 0325901和CHIR 99021的无血清培养基）改善了36个基因的甲基化状态。因此，这36个基因的DNA甲基化谱是评价猪iPSC的可行指标。创世纪51：763-776。© 2013 Wiley Periodicals，Inc.
Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor-intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)-specific hypomethylated loci (EShypo-T-DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso-4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso-4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso-622-14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum-free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc.
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DOI：
10.1089/clo.2008.0024
发表时间：
2008-09-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
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通讯作者：
Nagashima, Hiroshi
影响因子：
64.8
作者：
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影响因子：
2.5
作者：
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通讯作者：
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DOI：
10.1007/s12265-010-9233-3
发表时间：
2011-04-01
影响因子：
3.4
作者：
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通讯作者：
Izpisua Belmonte, Juan Carlos
影响因子：
64.5
作者：
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