Influence of Indocyanine Green on Hepatic Gd-EOB-DTPA Uptake: A Proof-of-Concept Study in Mice.
Influence of Indocyanine Green on Hepatic Gd-EOB-DTPA Uptake: A Proof-of-Concept Study in Mice.
复制标题
吲哚菁绿对肝脏 Gd-EOB-DTPA 摄取的影响：小鼠概念验证研究。
DOI：
10.1097/rli.0000000000000359
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Kiryu S.
中科院分区：
文献类型：
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作者：
Akai H;Yasaka K;Nojima M;Inoue Y;Ohtomo K;Kiryu S.
本研究旨在探讨吲哚青绿(ICG)对肝脏摄取Gd-EOB-DTPA的影响。材料与方法6只雌性C57BL6小鼠在注射Gd-EOB-DTPA前10分钟分别注射5 mg/kg、20 mg/kg ICG或磷酸盐缓冲盐水(对照组)，获得相同的三维梯度回波T1加权图像，绘制时间-强度曲线，测量肝实质峰值对比度(CRS)。我们研究了体温过低和体温正常的小鼠。所有实验条件下的CRS峰值均采用双因素方差分析和Bonferroni后检验。结果在低温小鼠中，3组的时间-强度曲线从5分钟到30分钟逐渐升高，30分钟后基本趋于平稳。CR值随着ICG注射剂量的增加而降低(对照组，5 mg/kg，20 mg/kg，分别为1.66±0.09，1.37±0.18，1.25±0.24)。在常温动物中，对照组和ICG5 mg/kg组的时间-强度曲线在注射后10~15min达到峰值，峰值CRS非常相似(对照组，ICG5 mg/kg分别为2.01±0.16，1.95±0.14)，然后强度逐渐下降，直到60min。与上述三组相比，ICG20 mg/kg组表现出较低的峰值CR(1.48±0.14)和较弱的强度下降至60min。注射剂量(P<；0.001)和实验温度(P<；0.001)对测量均有显著影响。结论吲哚青绿抑制肝脏对Gd-EOB-DTPA的摄取，影响Gd-EOB-DTPA增强磁共振成像的信号强度。这种抑制在体温过低的小鼠中更为明显。
ObjectivesThe aim of this study was to explore the influence of indocyanine green (ICG) on hepatic uptake of gadolinium ethoxybenzyldiethylenetriaminepenta-acetic acid (Gd-EOB-DTPA).Materials and MethodsGroups of 6 female C57BL6 mice were injected with 5 mg/kg ICG, 20 mg/kg ICG, or phosphate-buffered saline (control group) 10 minutes before the injection of Gd-EOB-DTPA; identical 3-dimensional gradient echo T1-weighted images were subsequently obtained to create time-intensity curves and to measure the peak contrast ratios (CRs) of liver parenchyma. We studied both hypothermic and normothermic mice. Peak CRs for all experimental conditions were evaluated, and among-group differences were assessed using 2-way factorial analysis of variance with Bonferroni post hoc testing.ResultsIn hypothermic mice, the time-intensity curves of the 3 groups gradually increased from 5 to 30 minutes and almost plateaued after 30 minutes. The peak CR decreased as the amount of injected ICG increased (control group, 5 mg/kg ICG, 20 mg/kg ICG: 1.66±0.09, 1.37±0.18, 1.25±0.24, respectively). In normothermic animals, the time-intensity curves of the control and ICG 5 mg/kg groups peaked 10 to 15 minutes after injection, the peak CRs were very similar (control group, 5 mg/kg ICG: 2.01±0.16, 1.95±0.14, respectively), and the intensities thereof then gradually fell until 60 minutes. Compared with these groups, the ICG 20 mg/kg group exhibited lower peak CR (1.48±0.14) and a weaker decrease in intensity to 60 minutes. Both the amount of ICG injected (P< 0.001) and the experimental temperature (P< 0.001) significantly affected the measurements.ConclusionsIndocyanine green inhibits the hepatic uptake of Gd-EOB-DTPA and affects the signal intensity upon Gd-EOB-DTPA–enhanced magnetic resonance imaging. Such inhibition was more obvious in hypothermic mice.