Recombination in large RNA viruses: Coronaviruses
Recombination in large RNA viruses: Coronaviruses
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DOI：
10.1006/smvy.1996.0046
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发表时间：
1996-12
期刊：
影响因子：
--
通讯作者：
Lai MM
中科院分区：
文献类型：
--
作者：
Lai MM
冠状病毒包含非常大的RNA基因组，其整个基因组以接近25%的非常高的频率进行重组。已证明病毒基因组之间以及缺陷干扰（DI）RNA与病毒RNA之间会发生重组。它为病毒RNA和DI RNA提供了进化工具，并可能解释冠状病毒基因组结构的多样性。冠状病毒进行重组的能力可能与其mRNA转录机制有关，该机制涉及不连续的RNA合成，表明病毒聚合酶的非进行性性质。诱变用作病毒基因组RNA诱变的工具。
Coronaviruses contain a very large RNA genome, which undergoes recombination at a very high frequency of nearly 25% for the entire genome. Recombination has been demonstrated to occur between viral genomes and between defective-interfering (DI) RNAs and viral RNA. It provides an evolutionary tool for both viral RNAs and DI RNA and may account for the diversity in the genomic structure of coronaviruses. The capacity of coronaviruses to undergo recombination may be related to its mRNA transcription mechanism, which involves discontinuous RNA synthesis, suggesting the nonprocessive nature of the viral polymerase. Recombination is used as a tool for the mutagenesis of viral genomic RNA.