MRT Letter: 3D Culture of Isolated Cells: A Fast and Efficient Method for Optimizing Their Histochemical and Immunocytochemical Analyses
MRT Letter: 3D Culture of Isolated Cells: A Fast and Efficient Method for Optimizing Their Histochemical and Immunocytochemical Analyses
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DOI：
10.1002/jemt.22470
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发表时间：
2015-04-01
影响因子：
2.5
通讯作者：
Mazzoleni, Giovanna
中科院分区：
文献类型：
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作者：
Berenzi, Angiola;Steimberg, Nathalie;Mazzoleni, Giovanna
三维(3D)培养系统和工程化细胞组织模型的快速发展引起了对新技术的日益增长的需求，使得能够对组织状结构中的细胞行为/形态进行微观观察，正如几位作者在过去十年中明确指出的那样。对于由悬浮培养的分离细胞的小聚集体组成的样品，通常很难生产出能够进一步成功地用于经典组织化学研究的最佳包埋制剂。在这项工作中，我们描述了一种新的、易于使用的、高效的方法，该方法能够在没有任何细胞应力或损伤的情况下，嵌入丰富的分离细胞/小3D细胞聚集体。至于组织包埋手术后，细胞块可以进一步适当地处理，进行有效的组织化学和免疫组织化学分析，使基于3D细胞的新生组织培养研究更具信息量和吸引力。Microsc.技术资源。2015年，78：249-254。(C)2015年威利期刊公司。
The rapid development of three-dimensional (3D) culture systems and engineered cell-based tissue models gave rise to an increasing need of new techniques, allowing the microscopic observation of cell behavior/morphology in tissue-like structures, as clearly signalled by several authors during the last decennium. With samples consisting of small aggregates of isolated cells grown in suspension, it is often difficult to produce an optimal embedded preparation that can be further successfully processed for classical histochemical investigations. In this work, we describe a new, easy to use, efficient method that enables to embed an enriched preparation of isolated cells/small 3D cell aggregates, without any cell stress or damage. As for after tissue-embedding procedures, the cellular blocks can be further suitably processed for efficient histochemical as well as immunohistochemical analyses, rendering more informative-and attractive-studies onto 3D cell-based culture of neo-tissues. Microsc. Res. Tech. 78:249-254, 2015. (c) 2015 Wiley Periodicals, Inc.