Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing
Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing
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DOI：
10.1007/s00251-010-0481-9
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发表时间：
2010-12-01
期刊：
影响因子：
3.2
通讯作者：
O'Connor, David H.
中科院分区：
文献类型：
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作者：
Budde, Melisa L.;Wiseman, Roger W.;O'Connor, David H.
非人灵长类动物的主要组织相容性复合体（MHC）I类等位基因与疾病易感性、抵抗力和消退相关。在这里，使用高分辨率焦磷酸测序，我们的特点是MHC I类转录表达的猕猴（MCM），非人灵长类动物的人口有限的MHC多样性。使用这种方法，我们确定了67个不同的MHC I类转录编码的7个最常见的MCM MHC I类单倍型，其中40（60%）跨越完整的开放阅读框架。这些结果使先前通过cDNA-PCR产物的克隆和桑格测序所定义的MHC I类序列的数量加倍，并且提供了用于MHC表征的快速、高通量和经济的方法。总体而言，这种方法显着扩大了我们的MCM单倍型的知识，并将促进疾病的发病机制和保护性细胞免疫的未来研究。
Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman primate population with restricted MHC diversity. Using this approach, we identified 67 distinct MHC class I transcripts encoded by the seven most frequent MCM MHC class I haplotypes, 40 (60%) of which span the complete open reading frames. These results double the number of MHC class I sequences previously defined by cloning and Sanger sequencing of cDNA-PCR products and provide a rapid, high-throughput, and economical method for MHC characterization. Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity.