Bone tissue engineering: recent advances and challenges.
Bone tissue engineering: recent advances and challenges.
复制标题
DOI：
10.1615/critrevbiomedeng.v40.i5.10
复制
发表时间：
2012
影响因子：
--
通讯作者：
Nukavarapu SP
中科院分区：
文献类型：
--
作者：
Amini AR;Laurencin CT;Nukavarapu SP
骨骼疾病和病症的全球发病率呈急剧上升趋势，预计到2020年将翻一番，特别是在老龄化与肥胖增加和体力活动不足相结合的人群中。工程化骨组织由于其无限供应和无疾病传播，已被视为传统骨移植物的潜在替代品。然而，由于一些限制或挑战，骨组织工程实践尚未进入临床实践。骨组织工程旨在通过生物材料、细胞和因子治疗的协同作用，诱导新的功能性骨再生。在这篇综述中，我们讨论了骨组织工程的基本原理，突出了这一领域的现状。此外，我们回顾了生物材料和细胞为基础的研究，以及用于促进骨再生的方法的最新进展。具体来说，我们讨论了广泛研究的生物材料支架，这些支架的微观和纳米结构特性，并纳入仿生性能和/或生长因子。此外，我们研究了各种细胞方法，包括使用间充质干细胞（MSC），胚胎干细胞（ESC），成体干细胞，诱导多能干细胞（iPSC）和富血小板血浆（PRP），以及它们的临床应用优势和局限性。最后，我们概述了骨组织工程领域面临的挑战，如缺乏足够的血管化在缺损部位，以及针对功能性骨组织工程的研究。这些挑战将推动该领域未来的研究。
The worldwide incidence of bone disorders and conditions has trended steeply upward and is expected to double by 2020, especially in populations where aging is coupled with increased obesity and poor physical activity. Engineered bone tissue has been viewed as a potential alternative to the conventional use of bone grafts, due to their limitless supply and no disease transmission. However, bone tissue engineering practices have not proceeded to clinical practice due to several limitations or challenges. Bone tissue engineering aims to induce new functional bone regeneration via the synergistic combination of biomaterials, cells, and factor therapy. In this review, we discuss the fundamentals of bone tissue engineering, highlighting the current state of this field. Further, we review the recent advances of biomaterial and cell-based research, as well as approaches used to enhance bone regeneration. Specifically, we discuss widely investigated biomaterial scaffolds, micro- and nano-structural properties of these scaffolds, and the incorporation of biomimetic properties and/or growth factors. In addition, we examine various cellular approaches, including the use of mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), adult stem cells, induced pluripotent stem cells (iPSCs), and platelet-rich plasma (PRP), and their clinical application strengths and limitations. We conclude by overviewing the challenges that face the bone tissue engineering field, such as the lack of sufficient vascularization at the defect site, and the research aimed at functional bone tissue engineering. These challenges will drive future research in the field.