Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle.
Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle.
复制标题
血管平滑肌中的油酸氧化和线粒体底物选择。
DOI：
10.1159/000051056
复制
发表时间：
2001
影响因子：
1.7
通讯作者：
Hardin,CD
中科院分区：
文献类型：
--
作者：
Allen,TJ;Hardin,CD
本研究的目的是确定长链脂肪酸相对于其他线粒体底物在活性血管平滑肌（VSM）中的贡献。猪颈动脉在含有0.71mMU-13 C-油酸（与白蛋白以6.8：1的比例结合）、5 mM 1-13 C-葡萄糖和1 mM乙酸盐的生理盐水溶液中，在存在或不存在5 mM肉毒碱的情况下，于37 ℃等长收缩6小时。使用谷氨酸盐的13 C-同位素分析测定底物氧化。虽然在生理浓度[0.5 mM（1：1）]下无法测量油酸的氧化，但在检查的较高浓度下，油酸氧化约占氧化的总底物的5%。虽然不显著，但肉毒碱增加油酸氧化至约8%，并导致内源性脂质氧化减少，其为总底物氧化的2-12%。由于孵育溶液中包含油酸，葡萄糖和乙酸盐的氧化没有显著变化。因此，我们得出结论，外源性长链脂肪酸是次要的贡献者底物氧化（约5%）在VSM相比，其他线粒体底物，如葡萄糖和乙酸，约占80%的底物氧化VSM。
The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-13 C-oleic acid (bound to albumin at a ratio of 6.8: 1), 5 mM 1-13 C-glucose and 1 mM acetate in the presence or absence of 5 mM carnitine for 6 h at 37 C. Substrate oxidation was determined using 13 C-isotopomer analysis of glutamate. Although oxidation of oleic acid could not be measured at physiological concentrations [0.5 mM (1: 1)], oleic acid oxidation was approximately 5% of the total substrates oxidized at the higher concentration examined. Although insignificant, carnitine increased oleic acid oxidation to approximately 8%, and resulted in a decrease in endogenous lipid oxidation, which was 2–12% of the total substrates oxidized. Oxidation of glucose and acetate did not significantly change due to the inclusion of oleic acid in the incubation solutions. Therefore, we conclude that exogenous long-chain fatty acids are minor contributors to substrate oxidation (approximately 5%) in VSM compared to other mitochondrial substrates, such as glucose and acetate, which account for approximately 80% of the substrates oxidized by VSM.