Local and systemic cytokine levels in relation to changes in vaginal flora
Local and systemic cytokine levels in relation to changes in vaginal flora
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DOI：
10.1086/499824
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发表时间：
2006-02-15
影响因子：
6.4
通讯作者：
Schwebke, JR
中科院分区：
文献类型：
--
作者：
Hedges, SR;Barrientes, F;Schwebke, JR
背景。细菌性阴道病（BV）与产科和妇科并发症和获得性传播疾病的风险增加有关。尽管如此，对这种疾病的发病机制知之甚少。采用酶联免疫吸附法测定正常菌群、中间菌群和BV患者阴道洗液和血清样本中白细胞介素(IL)-1b、肿瘤坏死因子- α、IL-6和IL-8的浓度（按Nugent标准测定）。细胞因子水平在感染中间菌群的妇女和感染细菌性阴道炎的妇女之间没有差异。携带中间菌群或细菌性阴道炎的妇女在阴道洗涤样品中IL-1 β的浓度明显高于携带正常菌群的妇女。通过阴道拭子标本的革兰氏染色检测，阴道洗涤样品中IL-1 β的存在与每高倍视野下的50 - 30种加德纳菌或普雷沃氏菌形态相关。乳酸菌和Mobiluncus菌种数量的变化不影响局部细胞因子水平。血清细胞因子水平不受阴道菌群变化的影响。中间菌群的妇女产生显著的细胞因子反应。与细菌性阴道炎相关的风险也可能影响到中间菌群的妇女，适当的治疗可以降低这种风险。
Background. Bacterial vaginosis ( BV) is associated with increased risk of obstetrical and gynecologic complications and acquisition of sexually transmitted diseases. Despite this, very little is known about the pathogenesis of this disease.Methods. Interleukin ( IL)-1b, tumor necrosis factor-alpha, IL-6, and IL-8 concentrations in vaginal wash and serum samples from women with normal flora, intermediate flora, and BV ( determined by Nugent criteria) were measured by enzyme-linked immunosorbent assay.Results. Cytokine levels were not different between women with intermediate flora and women with BV. Women with either intermediate flora or BV had significantly higher concentrations of IL-1 beta in vaginal wash samples than did women with normal flora. The presence of IL-1 beta in vaginal wash samples was associated with > 30 Gardnerella or Prevotella morphotypes per high-power field, as detected by Gram staining of vaginal swab specimens. Variation in the numbers of Lactobacillus and Mobiluncus species did not influence local cytokine levels. Serum cytokine levels were not influenced by any changes in vaginal flora.Conclusions. Women with intermediate flora generate significant cytokine responses. It is possible that the risks associated with BV may also affect women with intermediate flora and that appropriate treatment may reduce such risk.