Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo.
Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo.
复制标题
V beta 9 T 细胞对体外和体内次要淋巴细胞刺激抗原的差异反应性。
DOI：
10.1002/eji.1830210422
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发表时间：
1991
影响因子：
5.4
通讯作者：
Kanagawa,O
中科院分区：
文献类型：
--
作者：
Utsunomiya,Y;Kosaka,H;Kanagawa,O
单抗MR10-2是表达T细胞抗原受体Vβ9链的小鼠T细胞的特异性抗体。在许多不同品系的小鼠中，这种单抗与2%的外周T细胞发生反应，但在表达微小淋巴细胞刺激-1a抗原的小鼠中，由mR10-2识别的Vβ9+T细胞被删除。然而，我们在体外未能证明未激活的V-β9T细胞对mls-1a抗原的特异性反应。另一方面，用抗Vβ9(mr10-2)mabin体外激活的T细胞能够对mls-1a抗原产生反应。这些结果表明，V-β-9T细胞的MLS-1活性取决于T细胞的激活状态，提示未激活的初始T细胞和先前激活的T细胞之间存在差异激活要求。
The monoclonal antibody (mAb) MR10‐2 is specific for mouse T cells expressing the Vβ9 chain of T cell antigen receptor. This mAb reacts with 2% of peripheral T cells in a number of different strains of mice, but in mice expressing the minor lymphocyte stimulating (Mls)‐1aantigen, Vβ9+T cells recognized by MR10‐2 are deleted. However, we failed to demonstrate the Mls‐1aantigen‐specific response of naive, non‐activated Vβ9 T cellsin vitro.On the other hand, T cells activated initially with anti‐Vβ9 (MR10‐2) mAbin vitroare capable of responding to the Mls‐1aantigen. These results demonstrate that Mls‐1areactivity of Vβ9 T cells depends on the activation state of the T cells, suggesting a differential activation requirement between naive, non‐activated T cells and previously activated T cells.