Priming of DNA synthesis by diadenosine 5',5"'-P1,P4-tetraphosphate with a double-stranded octadecamer as a template and DNA polymerase alpha.
Priming of DNA synthesis by diadenosine 5',5"'-P1,P4-tetraphosphate with a double-stranded octadecamer as a template and DNA polymerase alpha.
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DOI：
10.1073/pnas.79.6.1791
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发表时间：
1982-03
影响因子：
11.1
通讯作者：
P. Zamecnik;E. Rapaport;E. Baril
中科院分区：
文献类型：
--
作者：
P. Zamecnik;E. Rapaport;E. Baril
二腺苷5‘，5“-P1，P4-四磷酸(Ap4A)在含有纯化的HeLa细胞DNA聚合酶α、脱氧腺苷三磷酸和双链合成十八聚体模板5’-d-(G-G-A-G-G-C-T-G-A-G-G-C-G-G-A-C-G-G)-d-5‘的体外体系中的DNA合成；该十八聚体序列是猴病毒40中DNA合成的起始区的一部分。在本实验条件下合成的短脱氧核酸链的第一个残基与Ap4A共价连接。该模板-引物系统可以启动新的脱氧核酸链，但不能将其延伸到A以外。T区。
Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) primes DNA synthesis in an in vitro system containing purified HeLa cell DNA polymerase alpha, deoxyadenosine triphosphate, and the double-stranded synthetic octadecamer template 5'-d-(G-G-A-G-G-C-T-T-T-T-T-T-G-G-A-G-G-C) (C-C-T-C-C-G-A-A-A-A-A-A-C-C-T-C-C-G)-d-5'; this octadecamer sequence is part of the origin region of DNA synthesis in simian virus 40. Ap4A is shown to be covalently linked to the first residue of the short deoxynucleotide chain synthesized under these experimental conditions. This template-primer system can initiate the new deoxynucleotide chain but cannot extend it beyond the A . T region.