Effects of composite antimicrobial peptides in weanling piglets challenged with deoxynivalenol: II. Intestinal morphology and function
Effects of composite antimicrobial peptides in weanling piglets challenged with deoxynivalenol: II. Intestinal morphology and function
复制标题
复合抗菌肽对脱氧雪腐镰刀菌烯醇攻击的断奶仔猪的影响：II。
DOI：
10.2527/jas.2013-6427
复制
发表时间：
2013-10-01
影响因子：
3.3
通讯作者：
Li, L.
中科院分区：
文献类型：
--
作者：
Xiao, H.;Tan, B. E.;Li, L.
脱氧雪腐镰刀菌烯醇（DON）影响动物和人类健康，靶向胃肠道。本研究旨在评价复合抗菌肽（CAP）对DON致仔猪肠道损伤的修复作用。选用28日龄断奶的杜×长×大约克郡仔猪28头，随机分为4组，每组7头，分别为阴性对照组、基础日粮（NC）组、基础日粮+0.4%复合抗菌肽（CAP）组、基础日粮+4mg/kg DON（DON）组和基础日粮+4mg/kg DON +0.4%CAP（DON + CAP）组。在7 d的适应期后，在治疗开始后第15和30 d采集血样，用于测定D-乳酸和二胺氧化酶的浓度。在研究结束时，屠宰所有仔猪以获得小肠，用于测定肠形态、上皮细胞增殖和哺乳动物雷帕霉素靶（mTOR）信号通路中的蛋白质表达。结果表明，DON使血清D-乳酸和二胺氧化酶浓度升高，CAP和DON + CAP处理的上述值分别低于NC和DON处理（P < 0.05）。CAP和DON + CAP组空肠和回肠绒毛高度/隐窝深度及回肠杯状细胞数均显著高于NC和DON组（P < 0.05）。DON + CAP组空肠和回肠的增殖细胞核抗原（PCNA）标记指数均高于DON组（P < 0.05）。DON降低空肠和回肠黏膜磷酸化Akt（蛋白激酶B）和mTOR蛋白的相对表达量（P < 0.05），降低空肠黏膜磷酸化4 E结合蛋白1（p-4 EBP 1）蛋白的相对表达量（P < 0.05），而CAP升高空肠黏膜p-4 EBP 1蛋白的相对表达量（P < 0.05）。结果表明，DON可增强肠道通透性，损伤绒毛，引起上皮细胞凋亡，抑制蛋白质合成; CAP可改善肠道形态，促进肠上皮细胞增殖和蛋白质合成，提示CAP可能对DON所致的肠道损伤具有修复作用。
Deoxynivalenol (DON) affects animal and human health and targets the gastrointestinal tract. The objective of this study was to evaluate the ability of composite antimicrobial peptides (CAP) to repair intestinal injury in piglets challenged with DON. A total of 28 piglets (Duroc x Landrace x Large Yorkshire) weaned at 28 d of age were randomly assigned to receive 1 of 4 treatments (7 pigs/treatment): negative control, basal diet (NC), basal diet + 0.4% composite antimicrobial peptide (CAP), basal diet + 4 mg/kg DON (DON), and basal diet + 4 mg/kg DON + 0.4% CAP (DON + CAP). After an adaptation period of 7 d, blood samples were collected on d 15 and 30 after the initiation of treatment for determinations of the concentrations of D-lactate and diamine oxidase. At the end of the study, all piglets were slaughtered to obtain small intestines for the determination of intestinal morphology, epithelial cell proliferation, and protein expression in the mammalian target of rapamycin (mTOR) signaling pathway. The results showed that DON increased serum concentrations of D-lactate and diamine oxidase, and these values in the CAP and DON + CAP treatments were less than those in the NC and DON treatments, respectively (P < 0.05). The villous height/crypt depth in the jejunum and ileum and the goblet cell number in the ileum in the CAP and DON + CAP treatments were greater than those in the NC and DON treatments (P < 0.05). The proliferating cell nuclear antigen (PCNA) labeling indexes for the jejunum and ileum in the DON + CAP treatment were greater than those in the DON treatment (P < 0.05). The DON decreased (P < 0.05) the relative protein expression of phosphorylated Akt (Protein Kinase B) and mTOR in the jejunal and ileal mucosa and of phosphorylated 4E-binding protein 1 (p-4EBP1) in the jejunal mucosa, whereas CAP increased (P < 0.05) the protein expression of p-4EBP1 in the jejunum. These findings showed that DON could enhance intestinal permeability, damage villi, cause epithelial cell apoptosis, and inhibit protein synthesis, whereas CAP improved intestinal morphology and promoted intestinal epithelial cell proliferation and protein synthesis, indicating that CAP may repair the intestinal injury induced by DON.