Theoretical and experimental evidence for eliminating persister bacteria by manipulating killing timing
Theoretical and experimental evidence for eliminating persister bacteria by manipulating killing timing
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DOI：
10.1093/femsle/fnw264
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发表时间：
2016-12-01
影响因子：
2.1
通讯作者：
Stiesch, M.
中科院分区：
文献类型：
--
作者：
Cogan, N. G.;Rath, H.;Stiesch, M.
细菌瞬时亚群的形成被称为持续体，是细菌用来逃避消除的最重要但最不被了解的机制之一。持久细胞似乎是生长缓慢的细菌，广泛地受到各种抗生素的保护。使用理论和实验方法，我们证明交替应用和停用抗生素可以是一种有效的治疗--只要准确地估计方案的时间。更具体地说，我们证明了基于理论预测的交替治疗的时机是通过实验观察得到证实的。这些结果支持了一大类理论研究，这些研究表明，即使没有完全了解生物学机制，这些模型也可以提供对系统属性的洞察。
Formation of a transient sub-population of bacteria, referred to as persisters, is one of the most important and least understood mechanisms that bacteria employ to evade elimination. Persister cells appear to be slow-growing bacteria that are broadly protected from a wide range of antibiotics. Using both theoretical and experimental methods, we show that alternating the application and withdrawal of antibiotics can be an effective treatment-as long as the timing of the protocol is estimated with precision. More specifically, we demonstrate that timing the alternating treatment based on theoretical predictions is confirmed using experimental observations. These results support a large class of theoretical studies that show that, even without complete understanding of the biological mechanisms, these models can provide insight into properties of the system.