PbWoxT1 mRNA from pear (Pyrus betulaefolia) undergoes long-distance transport assisted by a polypyrimidine tract binding protein
PbWoxT1 mRNA from pear (Pyrus betulaefolia) undergoes long-distance transport assisted by a polypyrimidine tract binding protein
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来自梨 (Pyrus betulaefolia) 的 PbWoxT1 mRNA 在聚嘧啶束结合蛋白的协助下进行长距离运输
DOI：
10.1111/nph.13793
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发表时间：
2016-04-01
期刊：
影响因子：
9.4
通讯作者：
Li, Tianzhong
中科院分区：
文献类型：
--
作者：
Duan, Xuwei;Zhang, Wenna;Li, Tianzhong
在嫁接的木本植物中，mRNAs在韧皮部的嫁接砧木和接穗之间进行长距离运输的机制知之甚少。我们在杜梨品种杜梨中发现了一个在韧皮部可运输的基因。它包含一个WUSCHEL相关同源框(WOX)结构域，因此被命名为WOX传输1(PbWoxT1)。PbWoxT1的548个碱基的片段在长距离运输中是至关重要的。PbWoxT1富含CuCu多嘧啶结构域，其mRNAs与多嘧啶结合蛋白PbPTB3相互作用。此外，在PbWoxT1或PbPTB3共表达系上嫁接的野生型烟草茎中PbWoxT1的表达显著增加，但在嫁接到PbWoxT1高表达砧木上的野生型烟草中PbWoxT1的表达没有显著增加，这表明PbPTB3介导了PbWoxT1 mRNA的长距离运输。我们提供了新的信息，增加了一种新的机制来解释WOX基因功能的非细胞自主方式，这丰富了我们对WOX基因在果树和其他物种中如何工作的理解。
Little is known about the mechanisms by which mRNAs are transported over long distances in the phloem between the rootstock and the scion in grafted woody plants. We identified an mRNA in the pear variety Du Li' (Pyrus betulaefolia) that was shown to be transportable in the phloem. It contains a WUSCHEL-RELATED HOMEOBOX (WOX) domain and was therefore named Wox Transport 1 (PbWoxT1). A 548-bp fragment of PbWoxT1 is critical in long-distance transport. PbWoxT1 is rich in CUCU polypyrimidine domains and its mRNAs interact with a polypyrimidine tract binding protein, PbPTB3. Furthermore, the expression of PbWoxT1 significantly increased in the stems of wild-type (WT) tobacco grafted onto the rootstocks of PbWoxT1 or PbPTB3 co-overexpressing lines, but this was not the case in WT plants grafted onto PbWoxT1 overexpressing rootstocks, suggesting that PbPTB3 mediates PbWoxT1 mRNA long-distance transport. We provide novel information that adds a new mechanism with which to explain the noncell-autonomous manner of WOX gene function, which enriches our understanding of how WOX genes work in fruit trees and other species.