Isolation and Characterization of a Mutant of a Methanol Yeast, Candida boidinii S2, with Higher Formaldehyde Productivity
Isolation and Characterization of a Mutant of a Methanol Yeast, Candida boidinii S2, with Higher Formaldehyde Productivity
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具有更高甲醛生产率的甲醇酵母突变体博伊丁假丝酵母 S2 的分离和表征
DOI：
10.1271/bbb1961.49.2699
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Hideaki Yamada
中科院分区：
文献类型：
--
作者：
Y. Tani;Y. Sakai;Hideaki Yamada
我们分离了一株利用甲醇的酵母菌，假丝酵母（Candida boidinii S2），它具有提高甲醛产量的特性。突变体筛选的程序包括；1)乙醇氧化酶在甲醇平板上的诱导，2)乙醇氧化酶在葡萄糖平板上的分解代谢失活，以及3)酒精氧化酶在菌落中的活性可视化。其中，菌株AOU-1的甲醛产率和甲醇生长率均比亲本菌株高1.7倍。甲醛产率高的原因是乙醇氧化酶活性高。在亲本菌株和突变菌株AOU-1之间，酶没有发生质的变化。突变菌株AOU-1的高活性可归因于数量变化和酶合成速率的变化。还讨论了突变体中分解代谢抑制和醇氧化酶失活。
We isolated a mutant strain of a methanol-utilizing yeast, Candida boidinii S2, which shows improved formaldehyde productivity. The procedure for mutant screening consisted of; 1) induction of alcohol oxidase on a methanol-plate, 2) catabolite inactivation of alcohol oxidase on a glucose-plate, and 3) visualization of alcohol oxidase activity in a colony. One of the mutants, strain AOU-1, showed 1.7 times higher formaldehyde productivity and a higher growth rate on methanol than the parent strain. The high formaldehyde productivity was proved to be due to the high alcohol oxidase activity. No qualitative change of the enzyme was detected between the parent strain and mutant strain AOU-1. The high activity of mutant strain AOU-1 could be attributed to a quantitative change and a change in the rate of enzyme synthesis. Catabolite repression and inactivation of alcohol oxidase in the mutant were also discussed.