Immunobiology of DC in NOD mice
Immunobiology of DC in NOD mice
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DOI：
10.1002/jlb.66.2.276
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发表时间：
1999-08-01
影响因子：
5.5
通讯作者：
Feili-Hariri, M
中科院分区：
文献类型：
--
作者：
Morel, PA;Vasquez, AC;Feili-Hariri, M
NOD小鼠在15至20周龄之间自发地发展为糖尿病，其之前是以淋巴细胞浸润为特征的胰岛炎，树突状细胞（DC）是最早浸润胰岛的细胞之一，并且它们与疾病的发病机制有关。我们的工作一直关注NOD小鼠中四种不同DC群体的详细表征：两种来源于在粒细胞-巨噬细胞集落刺激因子（GM-CSF）加白细胞介素-4（IL-4）或单独GM-CSF中培养的骨髓（BM）细胞，两种来源于Flt 3配体（FLt 3L）处理小鼠的脾脏，基于CD 8 α表达分离。NOD小鼠中这些DC亚群之间的表型和功能差异已被鉴定，此外，我们获得了较低产量的NOD BM衍生的DC，并且它们比来自糖尿病抗性株B10.BR的BM衍生的DC表达更高水平的细胞表面CD 40和IL-12 p40 mRNA。我们还研究了这些DC群体调节NOD小鼠糖尿病发展和进展的能力。
NOD mice spontaneously develop diabetes between 15 and 20 weeks of age, which is preceded by insulitis characterized by the infiltration of lymphocytes, Dendritic cells (DC) are among the first cells to infiltrate the islet and they have been implicated in the pathogenesis of the disease. Our work has been concerned with the detailed characterization of four distinct DC populations in NOD mice: two derived from bone marrow (BM) cells cultured in either granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4) or GM-CSF alone and two from the spleen of Flt3 ligand (FLt3L)-treated mice, isolated on the basis of CD8 alpha expression. Phenotypic and functional differences between these DC subsets in NOD mice have been identified, in addition, we obtained a lower yield of NOD BM-derived DC and they expressed higher levels of cell-surface CD40 and IL-12 p40 mRNA than BM-derived DC from the diabetes-resistant strain, B10.BR. We have also investigated the ability of these DC populations to modulate the development and progression of diabetes in NOD mice.