Necroptosis was found in a rat ischemia/reperfusion injury flap model
Necroptosis was found in a rat ischemia/reperfusion injury flap model
复制标题
大鼠缺血/再灌注损伤皮瓣模型发现坏死性凋亡
DOI：
10.1097/cm9.0000000000000005
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发表时间：
2019-01-05
影响因子：
6.1
通讯作者：
Wang, You-Bin
中科院分区：
文献类型：
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作者：
Liu, Hao;Zhang, Ming-Zi;Wang, You-Bin
背景：坏死性上睑下垂是一种新的细胞死亡形式，已被确定为导致细胞死亡的第三途径。本研究采用坏死他汀-1 （nec1）检测大鼠缺血/再灌注损伤皮瓣模型中是否存在坏死性上睑下垂。方法：选取雄性sd大鼠20只，随机分为对照组（CTL组）和Nec-1组。每个腹部皮瓣缺血3 h后再灌注。再灌注前后15分钟，CTL组腹腔注射磷酸缓冲盐水（PBS）， Nec-1组腹腔注射Nec-1。再灌注24小时后，将整个皮瓣平均分成54个切片。测量皮瓣血流灌注。每一行随机抽取一个样本。观察并检测细胞形态学变化、细胞凋亡、受体相互作用蛋白1 （receptor-interacting protein-1, RIP-1）表达及caspase-3活性。两组间比较采用独立t检验，P值<0.05为差异有统计学意义。结果：与CTL组相比，Nec-1组皮瓣存活时间更长，血流灌注更好，炎症浸润更少。CTL组存活皮瓣面积为70.88±10.28%，而Nec-1组存活皮瓣面积为80.56±5.40% （c-1 vs. CTL, t = -2.624, P < 0.05）。在部分行内，两组细胞凋亡有显著性差异，在“9 cm”、“7 cm”、“6 cm”和“5 cm”行内，Nec-1组细胞凋亡指数（AI）显著低于CTL组（Nec-1 vs. CTL, P < 0.05）。在第5 ~ 9 cm行，Nec-1组的RIP-1表达量明显低于CTL组（c-1 vs CTL, P < 0.05）。caspase-3活性无显著差异。结论：大鼠腹腔缺血再灌注损伤皮瓣模型存在坏死性上睑下垂。
Background: Necroptosis is a new form of cell death that has been identified as a third pathway causing cell death. In this study, necrostatin-1 (Nec-1) was used to determine whether necroptosis exists in a rat ischaemia/reperfusion injury flap model. Methods: In this study, twenty male Sprague-Dawley rats were divided randomly into two groups: a control group (CTL group) and a Nec-1 group. Each abdominal skin flap underwent 3 h of ischaemia and then reperfusion. Fifteen minutes before and after reperfusion, phosphate buffer saline (PBS) was administered intraperitoneally to the CTL group, while Nec-1 was administered intraperitoneally to the Nec-1 group. Twenty-four hours after reperfusion, the whole flap was divided equally into 54 sections. Flap blood perfusion was measured. One sample was taken randomly from each row. Morphological changes, apoptosis, receptor-interacting protein-1 (RIP-1) expression and caspase-3 activity were observed and detected. The measurements between the two groups were compared with the independent t-test, and a P value of <0.05 was considered statistically significant. Results: Compared to flaps in the CTL group, flaps in the Nec-1 group showed longer survival rates, better blood perfusion and less inflammatory infiltration. The total flap area considered to have survived was 70.88 ± 10.28% in the CTL group, whereas 80.56 ± 5.40% of the area was found to be living in the Nec-1 group (Nec-1 vs. CTL, t = –2.624, P < 0.05). For some rows, there were significant differences in cell apoptosis between the two groups, the apoptosis index (AI) in rows “9 cm”, “7 cm”, “6 cm” and “5 cm” was significantly lower in the Nec-1 group than that in the CTL group (Nec-1 vs. CTL, P < 0.05). RIP-1 expression was much lower in the Nec-1 group than that in the CTL group in rows “5 cm” to “9 cm” (Nec-1 vs. CTL, P < 0.05). No significant differences in caspase-3 activity were found. Conclusion: According to the results, necroptosis was present in a rat abdominal ischaemia/reperfusion injury flap model.