Nanomechanical Characterization of Bone Quality Depending on Tissue Age via Bimodal Atomic Force Microscopy
Nanomechanical Characterization of Bone Quality Depending on Tissue Age via Bimodal Atomic Force Microscopy
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DOI：
10.1007/s41871-023-00208-3
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发表时间：
2023-08
影响因子：
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通讯作者：
Jinha Kwon;Hanna Cho
中科院分区：
文献类型：
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作者：
Jinha Kwon;Hanna Cho
愈合过程中骨质量的表征对于植入手术的成功和患者的舒适度至关重要。在本研究中，对经过4周愈合期的骨植入物样本进行了研究。双峰原子力显微镜（AFM），同时获得的形态和弹性模量地图的新形成的和预先存在的骨区域内的样品。结果表明，新的骨基质具有较低的矿化水平，并呈现较大的，不均匀的矿物颗粒，表现出编织骨的属性。另一方面，旧的骨基质表现出更均匀和矿化的结构，这是板层骨的特征。新骨的总体弹性模量低于旧骨。双峰AFM进一步证实，新骨显示三个区域，包括未矿化，部分矿化和完全成熟的部分，这表明其组成的动荡变化。同时，旧骨显示出两个部分，包括部分矿化和成熟骨部分，这表示矿化的最后阶段。这项研究为新旧骨基质之间的形态学和纳米力学差异提供了有价值的见解，并提出了一种新的方法来研究骨愈合过程中不同阶段的骨质量。
Characterization of bone quality during the healing process is crucial for successful implantation procedures and patient comfort. In this study, a bone implant specimen that underwent a 4-week healing period was investigated. Bimodal atomic force microscopy (AFM) was employed to simultaneously obtain the morphology and elastic modulus maps of the newly formed and pre-existing bone regions within the sample. Results indicate that the new bone matrix possessed lower mineralization levels and presented larger, uneven mineral grains, exhibiting the attributes of a woven bone. On the other hand, the old bone matrix exhibited a more uniform and mineralized structure, which is characteristic of lamellar bones. The new bone had a lower overall elastic modulus than the old bone. Bimodal AFM further confirmed that the new bone displayed three regions comprising unmineralized, partially mineralized, and fully matured sections, which indicate a turbulent change in its composition. Meanwhile, the old bone exhibited two sections comprising partially mineralized and matured bone parts, which denote the final phase of mineralization. This study provides valuable insights into the morphological and nanomechanical differences between the old and new bone matrixes and presents a novel approach to investigate bone quality at different phases of the bone-healing process.