Role of mycelium and extracellular protein in the biodegradation of 2,4,6-trichlorophenol by Phanerochaete chrysosporium
Role of mycelium and extracellular protein in the biodegradation of 2,4,6-trichlorophenol by Phanerochaete chrysosporium
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菌丝体和胞外蛋白在黄孢原平革菌生物降解 2,4,6-三氯苯酚中的作用
DOI：
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发表时间：
1994
影响因子：
4.4
通讯作者：
G. Lewandowski
中科院分区：
文献类型：
--
作者：
P. Armenante;N. Pal;G. Lewandowski
在间歇式系统中研究了黄孢原毛革菌对2，4，6-三氯苯酚（2，4，6-TCP）的生物降解。在菌丝体悬浮液的实验中，发现在不存在木质素酶的情况下发生2，4，6-TCP的降解。在工艺结束时，氯离子以接近化学计量的量回收。在底物缺乏条件下，微生物没有保持其降解能力超过6天。无论是菌丝体还是细胞外蛋白都不能单独降解2，4，6-TCP;两者都需要完全降解。在将2，4，6-TCP暴露于从其菌丝体分离的培养物上清液的实验中，获得了可忽略的降解，并且没有回收氯离子。即使在上清液中添加过氧化氢作为可能的共底物时，也未观察到降解。在用从其上清液中分离的洗涤过的菌丝体进行的实验中，没有降解发生，直到菌丝体在孵育中5至6小时释放额外的细胞外蛋白。添加从其上清液中分离的洗涤过的菌丝体到活性培养物中也产生了与蛋白质释放相对应的降解速率的增加。蛋白质的释放与2，4，6-TCP的存在无关。添加放线菌酮抑制从头蛋白质的合成完全抑制了蛋白质的释放由菌丝体，导致没有2，4，6-TCP降解。添加含有高浓度胞外蛋白的培养上清液到活性培养物中产生2，4，6-TCP降解速率的增加。（250字处删节）
The biodegradation of 2,4,6-trichlorophenol (2,4,6-TCP) by Phanerochaete chrysosporium was studied in batch systems. In experiments with mycelial suspension, the degradation of 2,4,6-TCP was found to occur in the absence of ligninase. Chloride ion was recovered in nearly stoichiometric amounts at the end of the process. The microorganism did not retain its degradation ability for more than 6 days under substrate-deficient conditions. Neither the mycelium nor the extracellular protein alone could degrade 2,4,6-TCP; both were required for complete degradation to occur. In experiments in which 2,4,6-TCP was exposed to the culture supernatant separated from its mycelium, negligible degradation was obtained and no chloride ion was recovered. No degradation was observed even when the supernatant was supplemented with hydrogen peroxide as a possible cosubstrate. In experiments performed with washed mycelium separated from its supernatant, no degradation took place until the mycelium released additional extracellular protein 5 to 6 h into the incubation. Additions of washed mycelium separated from its supernatant to active cultures also produced an increase in the rate of degradation in correspondence with the protein release. The protein release was independent of the presence of 2,4,6-TCP. The addition of cycloheximide to inhibit the synthesis of de novo proteins completely suppressed the release of protein by the mycelium and resulted in no 2,4,6-TCP degradation. Additions of culture supernatants containing a high concentration of extracellular protein to active cultures produced an increase in the rate of 2,4,6-TCP degradation.(ABSTRACT TRUNCATED AT 250 WORDS)
DOI：
10.1016/s0021-9258(19)75982-1
发表时间：
1986-12
期刊：
The Journal of biological chemistry
影响因子：
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作者：
K. Hammel;B. Kalyanaraman;T. Kirk
通讯作者：
K. Hammel;B. Kalyanaraman;T. Kirk