Differentiation of isomeric 2'-, 3'- and 5'-deoxynucleosides by electron ionization and chemical ionization-linked scanning mass spectrometry.
Differentiation of isomeric 2'-, 3'- and 5'-deoxynucleosides by electron ionization and chemical ionization-linked scanning mass spectrometry.
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通过电子电离和化学电离联扫描质谱法区分异构体 2-、3- 和 5- 脱氧核苷。
DOI：
10.1002/bms.1200180804
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Schram,KH
中科院分区：
文献类型：
--
作者：
Reimer,ML;McClure,TD;Schram,KH
本文对2′-、3′-和5′-脱氧腺苷、2′-、3′-和5 ′-脱氧鸟苷、2 ′-、3 ′-和5 ′-脱氧黄嘌呤核苷以及2′-和5′-脱氧-2-氟腺苷的三甲基硅（TMS）衍生物进行了比较质谱检查。给出了11个脱氧核苷的低分辨率电子电离（EI）光谱中发现的主要碎片离子的一般汇编。以脱氧腺苷为模型化合物，报道了化学电离（CI）-碰撞活化（CA）子离子谱。离子结构和碎片化途径提出了这些离子的特点，每一个异构体。在异构体2′-、3′-和5′-嘌呤脱氧核苷之间存在显著的断裂差异。讨论了在这种分化中重要的十种离子的形成和结构。CI-CA链接的扫描光谱提供相对于EI质谱的互补结构信息。
A comparative mass spectral examination of the trimethylsilyl (TMS) derivatives of 2′‐, 3′‐ and 5′‐deoxyadenosine, 2′‐, 3′‐ and 5′‐deoxyguanosine, 2′‐, 3′‐ and 5′‐deoxyxanothosine and 2′‐ and 5′‐deoxy‐2‐fluoroadenosine is presented. A general compilation of the major fragment ions found in the low‐resolution electron ionization (EI) spectra of the eleven deoxynucleosides is given. Chemical ionization (CI)–collisional activation (CA) daughter ion spectra are reported using the deoxyadenosines as model compounds. Ion structures and fragmentation pathways are proposed for those ions characteristic of each of the isomers. Significant differences in fragmentation exist between the isomeric 2′‐, 3′‐ and 5′‐purine deoxynucleosides. The formation and structures of ten ions important in this differentiation are discussed. The CI–CA linked scan spectra provide complementary structural information relative to the EI mass spectra.