Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics
Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics
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通过重复序列和各种其他特征揭示日本慢生根瘤菌田间分离株的遗传相关性
DOI：
10.1128/aem.58.9.2832-2839.1992
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发表时间：
1992
影响因子：
4.4
通讯作者：
T. Asami
中科院分区：
文献类型：
--
作者：
K. Minamisawa;T. Seki;Shingo Onodera;M. Kubota;T. Asami
用4个来自B的DNA杂交探针对49个大豆慢生根瘤菌（Bradyrhizobium japonicum）菌株进行了鉴定。山茱萸nifDK特异性杂交清楚地将分离物分为两个不同的组。重复序列（RS）特异性杂交的多样性进行了观察，44个菌株来自41个结节分为33个不同的RS指纹组。聚类分析结果表明，nif和hup基因型与RS指纹图谱相关。我们发现多个带RS特异性杂交的两个菌株，不同于其他菌株的模式。这些结果表明，RS指纹图谱是一个有价值的工具，评估的遗传结构的土著B。japonicum种群
Forty-nine isolates of Bradyrhizobium japonicum indigenous to a field where soybeans were grown for 45 years without inoculation were characterized by using four DNA hybridization probes from B. japonicum. nifDK-specific hybridization clearly divided the isolates into two divergent groups. Diversity in repeated-sequence (RS)-specific hybridization was observed; 44 isolates derived from 41 nodules were divided into 33 different RS fingerprint groups. Cluster analysis showed that the RS fingerprints were correlated with the nif and hup genotypes. We found multiple bands of RS-specific hybridization for two isolates that differed from the patterns of the other isolates. These results suggest that RS fingerprinting is a valuable tool for evaluating the genetic structure of indigenous B. japonicum populations.