Defatted Sesame Seed Extract Reduces Brain Oedema by Regulating Aquaporin 4 Expression in Acute Phase of Transient Focal Cerebral Ischaemia in Rat
Defatted Sesame Seed Extract Reduces Brain Oedema by Regulating Aquaporin 4 Expression in Acute Phase of Transient Focal Cerebral Ischaemia in Rat
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DOI：
10.1002/ptr.4599
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发表时间：
2012-10-01
影响因子：
7.2
通讯作者：
Bu, Youngmin
中科院分区：
文献类型：
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作者：
Lee, Kyungjin;Jo, In-Young;Bu, Youngmin
脑水肿是脑组织体积的增加，已知与血管因素有关，包括血脑屏障(BBB)和血管通透性。除了神经保护外，抑制脑水肿也可以成为保护大脑免受缺血性损伤的一种方法。据报道，芝麻对心脑血管系统有多种有益作用。脱脂芝麻提取物(DSE)对大鼠大脑中动脉闭塞(TMCAo)模型的神经保护作用已有报道。目前的研究计划调查DSE的神经保护作用是否与脑水肿有关。采用tMCAo大鼠模型，观察脑组织含水量(BWC)和伊文思蓝(EB)渗漏情况。检测脑缺血后4h和2 4h水通道蛋白4(AQP4)、基质金属蛋白酶-2(MMP2)和MMP9的表达。体外酶谱检测MMPs活性的变化。DSE(30、100和300 mg/kg，P.O.)减少了BWC，但不会造成EB泄漏。DSE在缺血后4 h抑制AQP4的表达，但在24 h不抑制AQP4的表达。它对MMPs的表达和活性没有表现出任何影响。因此，DSE可能通过调节AQP4对脑缺血急性期的脑水肿起作用。版权所有(C)2012 John Wiley&Sons，Ltd.
Brain oedema is the volumetric increase of brain tissue and is known to be linked to vascular factors, including the bloodbrain barrier (BBB) and vascular permeability. Besides neuroprotection, inhibition of brain oedema also can be a method to protect the brain against ischaemic insult. Sesame is reported to have various beneficial effects on the cardiovascular and cerebrovascular systems. The neuroprotective effects of defatted sesame seed extract (DSE) in a transient middle cerebral artery occlusion (tMCAo) rat model were reported previously. The current study was planned to investigate whether the neuroprotective effects of DSE is related to brain oedema. The tMCAo rat model was used to investigate the brain water content (BWC) and Evans blue (EB) leakage. Aquaporin 4 (AQP4), matrix metalloproteinase (MMP)-2 and MMP-9 expressions at 4 and 24 h after ischaemia were analysed. In vitro zymography was performed to investigate the effects on MMPs activities. DSE (30, 100, and 300 mg/kg, p.o.) reduced BWC but not EB leakage. DSE inhibited AQP4 expression at 4 h but not at 24 h after ischaemia. It did not show any effects on MMPs expressions and activities. Therefore, DSE might be effective on brain oedema by AQP4 regulation during the acute phase of ischaemia. Copyright (c) 2012 John Wiley & Sons, Ltd.