BACTERIAL-GROWTH ON DISSOLVED ORGANIC-CARBON FROM A BLACKWATER RIVER
BACTERIAL-GROWTH ON DISSOLVED ORGANIC-CARBON FROM A BLACKWATER RIVER
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DOI：
10.1007/bf02014960
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发表时间：
1987-01-01
影响因子：
3.6
通讯作者：
RISLEY, R
中科院分区：
文献类型：
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作者：
MEYER, JL;EDWARDS, RT;RISLEY, R
通过超滤浓缩来自黑水东南部河流的不同标称分子量 (nMW) 的 DOC，并将其添加到过筛的河水中，以评估每种成分刺激细菌生长的能力。使用直接计数产生的细菌生物量的变化以及使用掺入 DNA 的 3H-胸苷来测量细菌生长。细菌生长和 DOC 用量在低 MW 富集 (< 1,000 nMW) 中最大，在中 MW 富集 (1,000-10,000 nMW) 中最小。高MW部分（>10,000 nMW）比中MW部分支持更多的生长，显然是因为较低MW的化合物与高MW耐火核心复合。来自清水山溪、北半球黑水河以及来自水栎和柳叶的渗滤液的低分子量 DOC 组分也比其他组分刺激更多的细菌生长。然而，来自这些其他来源的高分子量 DOC 的生物利用度不如来自黑水河的高分子量 DOC。细菌将黑水河 DOC 转化为细菌生物质，效率为 31%。以丰富的河流 DOC 为代价产生的细菌为原生动物和黑水河更高水平的微生物食物网提供了营养资源。
Different nominal molecular weight (nMW) fractions of DOC from a southeastern blackwater river were concentrated by ultrafiltration and added to sieved river water to assess each fraction''s ability to stimulate bacterial growth. Bacterial growth was measured using change in bacterial biomass from direct counts and using 3H-thymidine incorporated into DNA. Bacterial growth and amount of DOC used was greatest in the low MW enrichment (< 1,000 nMW) and least in the intermediate MW enrichment (1,000-10,000 nMW). The high MW fraction (>10,000 nMW) supported more growth than did the intermediate MW fraction, apparently because of lower MW compounds complexed with a high MW refractory core. The low MW fraction of DOC from a clearwater mountain stream, a boreal blackwater river, and leachate from water oak and willow leaves also stimulated more bacterial growth than did other fractions. However, the high MW DOC from these other sources was not as biologically available as high MW DOC from a blackwater river. Bacteria converted blackwater river DOC to bacterial biomass with an efficiency of 31%. Bacteria produced at the expense of abundant riverine DOC provide a trophic resource for protozoa and higher levels of the microbial food web of a blackwater river.