Essential role of the small subunit of thermostable glucose dehydrogenase from Burkholderia cepacia
Essential role of the small subunit of thermostable glucose dehydrogenase from Burkholderia cepacia
复制标题
洋葱伯克霍尔德菌耐热葡萄糖脱氢酶小亚基的重要作用
DOI：
10.1007/s10529-004-4582-0
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发表时间：
2004
影响因子：
2.7
通讯作者：
Masako Fujikawa Koji Sode
中科院分区：
文献类型：
--
作者：
Hideaki Yamaoka;S. Ferri;Masako Fujikawa Koji Sode
在大肠杆菌中共表达来自洋葱伯克霍尔德氏菌SM 4的热稳定葡萄糖脱氢酶（GDH）的γ-亚基和催化α-亚基，产生了12.7 U GDH活性mg−1蛋白。在γ-亚基的氨基末端鉴定出一个47个氨基酸的双精氨酸移位酶信号肽。在不存在γ亚基或γ亚基信号肽的情况下表达α亚基不能产生任何可检测的GDH蛋白或活性。γ-亚基可以是协助α-亚基多肽折叠成活性形式并易位至周质的分子伴侣样组分。
The co-expression in Escherichia coli of the γ-subunit and the catalytic α-subunit of the thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp. SM4 produced 12.7 U GDH activity mg−1 protein. A 47-amino acid, twin-arginine translocase signal peptide was identified at the amino terminus of the γ-subunit. The expression of the α-subunit in the absence of the γ-subunit or the γ-subunit signal peptide failed to produce any detectable GDH protein or activity. The γ-subunit may be a chaperone-like component that assists folding of the α-subunit polypeptide to the active form and its translocation to the periplasm.