Photoluminescent functionalized carbon dots for CRISPR delivery: synthesis, optimization and cellular investigation
Photoluminescent functionalized carbon dots for CRISPR delivery: synthesis, optimization and cellular investigation
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DOI：
10.1088/1361-6528/aafbf9
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发表时间：
2019-03-29
期刊：
影响因子：
3.5
通讯作者：
Karimi, Mahdi
中科院分区：
文献类型：
--
作者：
Hasanzadeh, Akbar;Radmanesh, Fatemeh;Karimi, Mahdi
使用成簇的规则间隔的短回文重复质粒（pCRISPR）的基因治疗减少了基因编辑中的错误，并防止产生在可用的基因组工程技术中已经看到的整合诱变。开发一种理想的和可追踪的纳米载体，它可以准确有效地将这种复合物转移到细胞质中，并促进向细胞核的旅程，是一个迷人的研究领域。采用微波辅助一步热解法制备了聚乙烯亚胺（PEI）功能化碳量子点（CD-PEI），其平均粒径约为3 nm。该CD-PEI显示出遗传物质的细胞内递送的良好潜力（类似于70%）。此外，这种用低分子量PEI（2kDa）进行被动表面改性的CD-PEI具有非常高的量子产率，高达40%，细胞毒性低。pCRISPR在HEK-293细胞中的表达率约为15%，这与原始PEI相当。此外，CD-PEI表现出良好的性质，例如高量子产率、生物相容性和可调谐的发射波长，表明光致发光功能化CD作为用于CRISPR递送的合适的、可追踪的纳米载体的潜在应用。
Gene therapy using clustered regularly interspaced short palindromic repeat plasmids (pCRISPR) reduces mistakes in gene editing and prevents engendering integrational mutagenesis that has been seen in available genome engineering technologies. Developing an ideal and traceable nanocarrier, which can accurately and efficiently transfer this complex into the cytosol and which facilitates the journey towards the nucleus, is a fascinating area of research. Polyethylenimine (PEI) functionalized carbon dots (CD-PEI) were fabricated by one-step microwave assisted pyrolysis with an average size around 3 nm. This CD-PEI showed good potential for intracellular delivery of genetic materials (similar to 70%). Also, this CD-PEI with passive surface modification with low molecular PEI (2 kDa) has a very high quantum yield, as high as 40% with low cytotoxicity. The expression rate of the pCRISPR was around 15% in the HEK-293 cell which is comparable with the pristine PEI. Furthermore, the CD-PEI demonstrated good properties, such as high quantum yield, biocompatibility and tunable emission wavelengths, suggesting the potential application of photoluminescent functionalized CDs as a suitable, traceable nanocarrier for CRISPR delivery.