Antibacterial efficacy and mechanisms of action of low power atmospheric pressure cold plasma: membrane permeability, biofilm penetration and antimicrobial sensitization
Antibacterial efficacy and mechanisms of action of low power atmospheric pressure cold plasma: membrane permeability, biofilm penetration and antimicrobial sensitization
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DOI：
10.1111/jam.13780
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发表时间：
2018-08-01
影响因子：
4
通讯作者：
Martines, E.
中科院分区：
文献类型：
--
作者：
Brun, P.;Bernabe, G.;Martines, E.
AimThe本研究的目的是确定两个临床相关的ESKAPE细菌，即铜绿假单胞菌和耐甲氧西林金黄色葡萄球菌大气压冷plasma.Methods和ResultsPlasma灭活的疗效和机制产生两个黄铜网格之间通过施加射频电场的氦气流。研究了反应性物种的细胞内生成、细胞膜的改变以及细菌在反硝化或生物膜生长中的失活。结果与常用的抗菌药物进行了比较。等离子体暴露在细菌中产生活性氧和氮物质，破坏膜的完整性并降低细菌负荷。在细菌灭活方面的功效与抗生素相当，但表现出更快的杀灭率。等离子体的抗菌作用与抗生素协同增加，并且在反复暴露后没有减弱，表明细菌耐药性没有发展。结论通过产生活性物质，冷等离子体改变了细胞膜，并有效地灭活了悬浮液和生物膜中的临床重要细菌。它作为单独使用或与抗微生物药物组合使用以控制微生物感染和防止抗性细菌菌株的选择的有效策略而出现。
AimThe objective of this study was to determine the efficacy and mechanisms of inactivation of two clinically relevant ESKAPE bacteria namely Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus by atmospheric pressure cold plasma.Methods and ResultsPlasma was generated between two brass grids by applying a radiofrequency electric field to a flow of helium. Intracellular generation of reactive species, alterations in cell membrane, and inactivation of bacteria in planktonic or biofilm growth were studied. Results were compared with commonly used antimicrobial drugs. Plasma exposure generated reactive oxygen and nitrogen species in bacteria, disrupted membrane integrity and reduced bacterial load. The efficacy in bacterial inactivation was comparable to antibiotics but exhibited a quicker killing rate. The antibacterial effect of plasma synergistically increased in association with antibiotics and did not diminish over repeated exposures, suggesting no development in bacterial resistance.ConclusionsThrough generation of reactive species, cold plasma altered cell membrane and effectively inactivated clinically important bacteria, both in suspension and in biofilms.Significance and Impact of the StudyAs cold plasma damages different targets in bacterial cells, it emerges as an effective strategy used alone or in combination with antimicrobial drugs to control microbial infections and prevent the selection of resistant bacterial strains.