Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India.
Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India.
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DOI：
10.1016/j.gdata.2015.03.003
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
Dudhagara P
中科院分区：
文献类型：
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作者：
Ghelani A;Patel R;Mangrola A;Dudhagara P
利用细菌标签编码的Flx扩增子焦磷酸测序(BTEFAP)，从印度图尔西沙姆温泉中检索到的5.78Mb元基因组序列中推导出细菌的分类描述。Metagenome包含10,893个16S rDNA序列，MG-RAST服务器对其进行分析，以生成细菌的全面图谱。元基因组数据可在EBI上的EBI元基因组数据库下获得，登录号：ERP009559。后基因组序列占细菌来源的98.2%，真核生物的1.5%，未鉴定的0.3%。温泉超基因组共发现细菌16门，隶属97科287种。种类最多的门是藻类(65.38%)、变形杆菌(21.21%)和未分类细菌(10.69%)。其中以消化链球菌科(37.33%)、梭状芽孢杆菌科(23.36%)和肠杆菌科(16.37%)为最高。常见菌种为双发酵梭菌(17.47%)、垃圾梭菌(13.93%)和未培养细菌(10.15%)。我们的数据提供了有关温泉细菌的新信息，并阐明了它们的丰度、多样性、分布和共存生物。
A taxonomic description of bacteria was deduced from 5.78 Mb metagenomic sequence retrieved from Tulsi Shyam hot spring, India using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome contained 10,893 16S rDNA sequences that were analyzed by MG-RAST server to generate the comprehensive profile of bacteria. Metagenomic data are available at EBI under EBI Metagenomics database with accession no. ERP009559. Metagenome sequences represented the 98.2% bacteria origin, 1.5% of eukaryotic and 0.3% were unidentified. A total of 16 bacterial phyla demonstrating 97 families and 287 species were revealed in the hot spring metagenome. Most abundant phyla were Firmicutes (65.38%), Proteobacteria (21.21%) and unclassified bacteria (10.69%). Whereas, Peptostreptococcaceae (37.33%), Clostridiaceae (23.36%), and Enterobacteriaceae (16.37%) were highest reported families in metagenome. Ubiquitous species were Clostridium bifermentans (17.47%), Clostridium lituseburense (13.93%) and uncultured bacterium (10.15%). Our data provide new information on hot spring bacteria and shed light on their abundance, diversity, distribution and coexisting organisms.