Mammalian cyclin/PCNA (DNA polymerase delta auxiliary protein) stimulates processive DNA synthesis by yeast DNA polymerase III.
Mammalian cyclin/PCNA (DNA polymerase delta auxiliary protein) stimulates processive DNA synthesis by yeast DNA polymerase III.
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DOI：
10.1093/nar/16.14.6297
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发表时间：
1988-07
影响因子：
14.9
通讯作者：
P. Burgers
中科院分区：
文献类型：
--
作者：
P. Burgers
人细胞周期蛋白/PCNA（增殖细胞核抗原）在结构、功能和免疫上与小牛胸腺DNA聚合酶辅助蛋白同源。该辅助蛋白已被研究为酿酒酵母核DNA聚合酶的刺激因子。犊牛细胞周期蛋白/PCNA可将DNA聚合酶III复制高模板/引物比率的模板（如poly(dA)）的能力提高10倍以上。益生元(dT)(40:1)。刺激程度随着模板/引物比例的增加而增加。在高模板/引物比下，即低引物密度下，细胞周期蛋白/PCNA极大地促进了DNA聚合酶III的DNA合成。低模板/引物比例（如poly(dA)）。在寡核苷酸（dT）（2.5:1）的情况下，细胞周期蛋白/PCNA的加入仅能最低限度地增加DNA聚合酶III的活性，但仍能观察到对总DNA合成的几倍刺激。这表明细胞周期蛋白/PCNA也可能增加DNA聚合酶III与模板引物的有效结合，并稳定模板引物聚合酶复合物。酵母DNA聚合酶I和II的活性不受细胞周期蛋白/PCNA添加的影响。这些结果加强了酵母DNA聚合酶III在功能上类似于哺乳动物DNA聚合酶delta的假设。
Human cyclin/PCNA (proliferating cell nuclear antigen) is structurally, functionally, and immunologically homologous to the calf thymus auxiliary protein for DNA polymerase delta. This auxiliary protein has been investigated as a stimulatory factor for the nuclear DNA polymerases from S. cerevisiae. Calf cyclin/PCNA enhances by more than ten-fold the ability of DNA polymerase III to replicate templates with high template/primer ratios, e.g. poly(dA).oligo(dT) (40:1). The degree of stimulation increases with the template/primer ratio. At a high template/primer ratio, i.e. low primer density, cyclin/PCNA greatly increases processive DNA synthesis by DNA polymerase III. At low template/primer ratios (e.g. poly(dA).oligo(dT) (2.5:1), where addition of cyclin/PCNA only minimally increases the processivity of DNA polymerase III, a several-fold stimulation of total DNA synthesis is still observed. This indicates that cyclin/PCNA may also increase productive binding of DNA polymerase III to the template-primer and stabilize the template-primer-polymerase complex. The activity of yeast DNA polymerases I and II is not affected by addition of cyclin/PCNA. These results strengthen the hypothesis that yeast DNA polymerase III is functionally analogous to the mammalian DNA polymerase delta.