ENHANCING DNA DETECTION SENSITIVITY THROUGH A TWO-STEP ENRICHMENT METHOD WITH MAGNETIC BEADS AND DROPLET EVAPORATION
ENHANCING DNA DETECTION SENSITIVITY THROUGH A TWO-STEP ENRICHMENT METHOD WITH MAGNETIC BEADS AND DROPLET EVAPORATION
复制标题
通过磁珠和液滴蒸发的两步富集方法提高 DNA 检测灵敏度
DOI：
10.1080/00032710903502140
复制
发表时间：
2010-01-01
影响因子：
2
通讯作者：
Li, Jiong
中科院分区：
文献类型：
--
作者：
Du, Xing-Li;Duan, De-Min;Li, Jiong
通过两步富集法建立了一种简便、灵敏的DNA检测方法。在大体积(1mL)中选择性地分离微量靶DNA，并用DNA修饰的磁珠经变性缩合成小体积(5亩L)。然后，将预富集的目标DNA溶液(1亩L)转移到光滑的疏水表面，通过自然固着液滴蒸发进一步富集目标DNA。使用该方法，目标DNA的荧光检测灵敏度可提高3个数量级，在2小时内可检测到低至3.91pM的目标DNA。
A simple and sensitive DNA detection method has been developed through a two-step enrichment process. Trace amount of target DNA in a large volume (1 mL) is selectively separated and condensed with DNA-modified magnetic beads into a small volume (5 mu L) by denaturalization. Then, the pre-enriched target DNA solution (1 mu L) is transferred onto a smooth hydrophobic surface, where the target DNA is further-enriched by natural sessile droplet evaporation. Using this method, the fluorescence detection sensitivity of the target DNA can be enhanced by 3 orders of magnitude and as low as 3.91pM of the target DNA can be detected within 2 hours.