Atoh7 promotes retinal Müller cell differentiation into retinal ganglion cells
Atoh7 promotes retinal Müller cell differentiation into retinal ganglion cells
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DOI：
10.1007/s10616-014-9777-1
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发表时间：
2016-03
期刊：
影响因子：
2.2
通讯作者：
Wei‐tao Song;Q. Zeng;X. Xia;K. Xia;Q. Pan
中科院分区：
文献类型：
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作者：
Wei‐tao Song;Q. Zeng;X. Xia;K. Xia;Q. Pan
青光眼是由于视网膜神经节细胞死亡而导致的主要眼部疾病之一。越来越多的证据表明，视网膜Müller细胞表现出视网膜祖细胞的特征，并在一定条件下可以在受损的视网膜中分化为神经元。然而，从视网膜Müller细胞分化的神经节细胞的数量福尔斯远远低于治疗需求。本研究旨在通过将Atoh 7导入视网膜Müller细胞去分化的干细胞中来促进视网膜Müller细胞向神经节细胞的分化。分离大鼠视网膜Müller细胞，将其去分化为干细胞，用PEGFP-N1或PEGFP-N1-Atoh 7载体转染，进一步诱导其向神经节细胞分化。从Atoh 7转染的干细胞分化的神经节细胞的比例显著高于对照转染或未转染的细胞。总之，Atoh 7促进视网膜Müller细胞分化为视网膜神经节细胞。这可能为通过促进视神经再生进行青光眼基因治疗开辟新的途径。
Glaucoma is one of the leading eye diseases due to the death of retinal ganglion cells. Increasing evidence suggests that retinal Müller cells exhibit the characteristics of retinal progenitor cells and can differentiate to neurons in injured retinas under certain conditions. However, the number of ganglion cells differentiated from retinal Müller cells falls far short of therapeutic needs. This study aimed to promote the differentiation of retinal Müller cells into ganglion cells by introducing Atoh7 into the stem cells dedifferentiated from retinal Müller cells. Rat retinal Müller cells were isolated and dedifferentiated into stem cells, which were transfected with PEGFP-N1 or PEGFP-N1-Atoh7 vector, and then further induced to differentiate into ganglion cells. The proportion of ganglion cells differentiated from Atoh7-tranfected stem cells was significantly higher than that of control transfected or untransfected cells. In summary, Atoh7 promotes the differentiation of retinal Müller cells into retinal ganglion cells. This may open a new avenue for gene therapy of glaucoma by promoting optic nerve regeneration.