Visualization of enzymatic reaction in a microfluidic channel using chemical imaging sensor
Visualization of enzymatic reaction in a microfluidic channel using chemical imaging sensor
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DOI：
10.1016/j.electacta.2013.08.089
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发表时间：
2013-12-15
影响因子：
6.6
通讯作者：
Yoshinobu, Tatsuo
中科院分区：
文献类型：
--
作者：
Miyamoto, Ko-ichiro;Hirayama, Yuji;Yoshinobu, Tatsuo
化学成像传感器是一种基于生物传感器的化学传感器，能够可视化化学物种的空间分布。在这项研究中，我们将化学成像传感器应用于无染料的方法，在微流体通道中可视化酶反应。载有酶分子的微凝胶珠被捕获在微通道中。从上游注入酶的底物，然后随着pH的变化在下游检测酶反应的产物。成功地可视化了酶促反应引起的pH分布的时空变化。微通道内化学成像的可能性也表明了潜在的应用，例如，色谱法。(C)2013爱思唯尔有限公司保留所有权利。
The chemical imaging sensor is a semiconductor-based chemical sensor which is capable of visualizing the spatial distribution of chemical species. In this study, we applied the chemical imaging sensor to a dye-free method of visualizing an enzymatic reaction in a microfluidic channel. Microgel beads loaded with enzyme molecules were trapped in the microchannel. The substrate of the enzyme was injected from the upstream, and then the product of enzymatic reaction was detected in the downstream as pH change. The spatiotemporal change of pH distribution caused by the enzymatic reaction was successfully visualized. The possibility of chemical imaging inside a microchannel also suggests potential application, for example, to chromatography. (C) 2013 Elsevier Ltd. All rights reserved.