Application of Bacterial DNA Fingerprinting in Crude Oil for Evaluating the Reservoir-Part I*
Application of Bacterial DNA Fingerprinting in Crude Oil for Evaluating the Reservoir-Part I*
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原油中细菌 DNA 指纹图谱在油藏评价中的应用-第一部分*
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
F. Boukadi
中科院分区：
文献类型：
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作者：
A. Hayatdavoudi;N. Chegenizadeh;A. Christoserdov;R. Bajpai;F. Boukadi
一种新的、灵敏的细菌DNA指纹技术--聚合酶链式反应扩增的16S rRNA基因变性梯度凝胶电泳(DGGE)--被用来对从一家运营公司获得的六个原油样本中发现的细菌进行鉴定和分类。这些样本取自两口不同的油井，据推测是在同一油层钻探的。对油样中细菌群落进行指纹分析的步骤为：从油样中提取DNA，DNA浓缩，从油样中提取的DNA中扩增出16S rRNA基因片段，用DGGE分离片段，对凝胶中的DNA条带进行测序，最后鉴定这些条带所对应的细菌。所有原油样品经DNA浓缩后均证实了细菌DNA的存在。根据从两口井中鉴定出的细菌，其中一口井似乎从与草原相关的淡水环境中排出石油，而第二口井从沿海海洋环境中排出石油。这一发现与色谱图(指纹)形成了鲜明的对比，后者表明两个油样是相同的。
A novel, sensitive technique for bacterial DNA fingerprinting – denaturing gradient gel electrophoresis (DGGE) of PCR amplified 16S rRNA genes – has been used to identify and classify bacteria found in six crude oil samples obtained from an operating company. The samples were taken from two separate wells, presumably drilled in the same reservoir. The procedure of fingerprinting bacterial communities present in the oil samples was as follows: DNA extraction from oil, DNA concentration, PCR amplification of 16S rRNA gene fragments from DNA isolated from oil, separation of the fragments by DGGE, sequencing DNA bands present in the gels, and finally identification of bacteria to which the these bands correspond. The existence of bacterial DNA in all the crude oil samples was proven after DNA concentration. Based on the bacteria identified from the two wells, it appears that one well drained oil from a fresh water environment associated with grasslands, whereas the second well drained oil from a coastal marine environment. This finding is in a sharp contrast to Chromatograms (fingerprints) which indicates both oil samples are the same.