Immunological detection of wood decay fungi - An overview of techniques developed from 1986 to the present

Immunological detection of wood decay fungi - An overview of techniques developed from 1986 to the present
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DOI:
10.1016/s0964-8305(97)00017-6
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发表时间:
1997-03-01
影响因子:
4.8
通讯作者:
Clausen, CA
Clausen, CA
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
Clausen, CA

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在过去的十年中,已经开发出利用抗体靶向真菌抗原来检测早期木材腐烂的体外和原位诊断方法。抗体是检测真菌生物降解的潜在理想探针,因为它们是特异性的,可以量化复杂结构(如木材)内的真菌抗原。针对各种真菌成分的多克隆和单克隆抗体已分别用于免疫印迹、酶免疫测定、颗粒凝集测定和色谱“试纸”测定。本文综述了1986年以来林产品免疫诊断领域所面临的挑战和取得的进展。Elsevier Science Limited出版。
During the past decade, diagnostic methods for detecting incipient wood decay in vitro and in situ have been developed using antibodies to target fungal antigens. Antibodies are potentially ideal probes for detecting fungal biodeterioration because they are specific and can quantitate fungal antigens within a complex structure such as wood. Both polyclonal and monoclonal antibodies to various fungal components have been utilized separately and in concert for immunoblotting, enzyme immunoassays, particle agglutination assays, and chromatographic 'dipstick' assays. This paper provides an overview of the challenges encountered and progress made in the field of forest products immunodiagnostics since 1986. Published by Elsevier Science Limited.