An improved pollen collection and cryopreservation method for highly recalcitrant tropical fruit species of mango (Mangifera indica L.) and litchi (Litchi chinensis Sonn.).
An improved pollen collection and cryopreservation method for highly recalcitrant tropical fruit species of mango (Mangifera indica L.) and litchi (Litchi chinensis Sonn.).
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一种针对芒果（Mangifera indica L.）和荔枝（Litchi chinensis Sonn.）等高度顽固热带水果品种的花粉采集和冷冻保存方法的改进。
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
S. Rajan
中科院分区：
文献类型：
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作者：
R. Chaudhury;S. Malik;S. Rajan
设计了一种使用有机溶剂环己烷从芒果（Mangifera indica L.）和荔枝（Litchi chinensis Sonn.）新鲜开裂的花药中收集花粉的改进方法。使用这种方法，可以收集并储存足以进行大规模授粉的花粉量以供将来使用。为这两种水果品种成功设计了在可行条件下将花粉从采集地点（田间种质库）长距离运输到冷冻实验室的技术。冷冻保存已成功应用于实现长达四年的长期花粉储存。使用体外发芽、荧光染色反应（FCR）方法和田间授粉后的坐果来测试花粉活力。在重新测试中，不同芒果和荔枝品种的四年冷冻花粉显示出与新鲜对照花粉一样高的活力百分比。利用所开发的技术，180多个芒果品种和19个荔枝品种的花粉已被储存在低温库中，从而促进了长期育种计划。
An improved method for pollen collection from freshly dehiscing anthers of mango (Mangifera indica L.) and litchi (Litchi chinensis Sonn.) using the organic solvent cyclohexane has been devised. Using this method pollen quantity sufficient for large scale pollinations could be collected and stored for future use. Transport of pollen in viable conditions over long distances, from site of collection (field genebank) to cryolab was successfully devised for both these fruit species. Cryopreservation was successfully applied to achieve long-term pollen storage over periods of up to four years. Pollen viability was tested using in vitro germination, the fluorochromatic reaction (FCR) method and by fruit set following field pollination. On retesting, four year cryostored pollen of different mango and litchi varieties showed high percentage viability as good as fresh control pollens. Pollens of more than 180 cultivars of mango and 19 cultivars of litchi have been stored in the cryogenebank using the technology developed, thus facilitating breeding programmes over the long-term.