Tall fescue endophyte detection : Commercial immunoblot test kit compared with microscopic analysis
Tall fescue endophyte detection : Commercial immunoblot test kit compared with microscopic analysis
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高羊茅内生菌检测：商业免疫印迹检测试剂盒与显微镜分析的比较
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
J. A. Stuedemann
中科院分区：
文献类型：
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作者：
E. E. Hiatt;N. Hill;J. Bouton;J. A. Stuedemann
一种可靠、高效和准确的内生真菌Neotyphdium coenphalum(Morgan-Jones and Gams)Glenn、Bacon和Hanlin Comb存在的检测方法。11月高羊茅(Festuca arundinacea Schreb.)种子和植物组织将有利于高羊茅育种和种子批次分析。本实验是为了确定新伤寒杆菌免疫印迹检测试剂盒(农业有限公司，沃特金斯维尔，佐治亚州)的准确性、可靠性和重复性。组织免疫印迹与组织学染色进行了比较，然后对温室生长试验中的高羊茅分蘖、田间种植的间隔株、已建立的田间围场和不同高羊茅种子地进行了显微分析。用这两种方法对感染内生菌和不感染内生菌的高羊茅种群进行了评价。无论人群的感染水平、检测的组织类型或进行检测的技术人员，这两种方法获得的结果都是相似的。免疫印迹检测试剂盒准确可靠，易于容纳大量样本。
A reliable, efficient, and accurate detection method for presence of the endophytic fungus Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon, and Hanlin comb. nov. in tall fescue (Festuca arundinacea Schreb.) seed and plant tissue would be beneficial for tall fescue breeding and seed lot analysis. This experiment was conducted to determine the accuracy, reliability, and reproducibility of the Phytoscreen Neotyphodium immunoblot detection kit (Agrinostics Ltd. Co., Watkinsville, GA). Tissue immunoblot was compared with histological staining followed by microscopic analysis on tall fescue tillers from a greenhouse grow-out test, field grown spaced plants, established field paddocks, and different tall fescue seed lots. Endophyte-infected and endophyte-free tall fescue populations were evaluated by both methods. Results obtained by both methods were similar regardless of the infection level of the population, type of tissue assayed, or the technician that conducted the assay. The immunoblot detection kit was accurate and reliable and readily accommodated large numbers of samples.