Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets
Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets
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DOI：
10.1002/anie.200804326
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发表时间：
2009-01-01
影响因子：
16.6
通讯作者：
Andersson-Svahn, Helene
中科院分区：
文献类型：
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作者：
Joensson, Haakan N.;Samuels, Michael L.;Andersson-Svahn, Helene
发现少数：细胞表面蛋白是有用的疾病生物标志物，但目前的高通量方法仅限于检测表达数百种以上蛋白质的细胞。微流体液滴中的酶促扩增（见图）是一种高通量方法，用于检测和分析在单个人类细胞上表达水平非常低的细胞表面生物标志物。液滴光学标签允许同时分析多个样品。
Finding the few: Cell‐surface proteins are useful disease biomarkers, but current high‐throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high‐throughput method for detection and analysis of cell‐surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.