Effects of Nitrate Reductase Inhibitor Na_2WO_4 on Nitrate Accumulation in Oilseed Rape
Effects of Nitrate Reductase Inhibitor Na_2WO_4 on Nitrate Accumulation in Oilseed Rape
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作者：
Q. Wei
【摘要】：通过无土培养(营养液)试验,研究硝酸盐还原酶抑制剂Na2WO4对2个不同品种(高硝态氮积累型‘中双6号’和低硝态氮积累型‘红油3号’)油籽部分器官(包括根、叶、叶柄)硝酸还原酶(NR)活性和NO3--N含量的影响。 结果表明，Na2WO4最适浓度为1.0 mmol·L-1；与对照（CK）相比，Na2WO4显着降低了油菜根部NR活性和NO3--N吸收率，但NO3--N含量显着增加。 与低硝积累型‘红油3号’相比，缓蚀剂效果显着。添加Na2WO4和不添加Na2WO4处理后，叶片和叶柄的NR活性没有显着差异，而Na2WO4显着降低了油菜叶柄中NO3--N含量，且处理后叶片中NO3--N含量基本稳定，尤其是‘红油3号’。
A soilless culture(nutrient solution) experiment was conducted to study the effects of nitrate reductase inhibitor Na2WO4 on nitrate reductase(NR) activity and NO3--N content in some organs(including root,leaf,petiole) of two different cultivars(high nitrate-N accumulation type 'Zhongshuang 6' and low nitrate-N accumulation type 'Hongyou 3') oilseed rape(Brassica napus L.) at seedling stage.Results showed that the most appropriate concentration of Na2WO4 was 1.0 mmol·L-1;Na2WO4 significantly decreased NR activity and NO3--N absorption rate in the root of oilseed rape comparing with the control(CK),however,significantly increasing in NO3--N content.High nitrate accumulation type 'Zhongshuang 6' had higher inhibiter efficiency comparing with low nitrate accumulation type 'Hongyou 3'.There were no significant difference on NR activity in leaf and petiole with or without Na2WO4,in contrast,Na2WO4 significantly decreased NO3--N content in the petiole of oilseed rape,and NO3--N content in the leaf was almost stable under it treatment,especially in 'Hongyou 3'.