Cerium oxide nanoparticles protect gastrointestinal epithelium from radiation-induced damage by reduction of reactive oxygen species and upregulation of superoxide dismutase 2
Cerium oxide nanoparticles protect gastrointestinal epithelium from radiation-induced damage by reduction of reactive oxygen species and upregulation of superoxide dismutase 2
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DOI：
10.1016/j.nano.2010.01.010
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发表时间：
2010-10-01
影响因子：
5.4
通讯作者：
Baker, Cheryl H.
中科院分区：
文献类型：
--
作者：
Colon, Jimmie;Hsieh, Nelson;Baker, Cheryl H.
研究了稀土氧化铈(CeO2)纳米颗粒对胃肠道上皮的辐射防护作用。在单次剂量辐射照射前24小时，用不同浓度的CeO2纳米粒子对正常人结肠细胞(CRL 1541)进行预处理，以剂量依赖的方式减少活性氧的产生和增加超氧化物歧化酶2(SOD2)的表达，从而提供对辐射诱导的细胞死亡的保护。在随后的实验中，裸鼠在接受单次照射之前，先在腹壁内注射CeO2纳米颗粒，然后进行预处理。免疫组织化学分析显示，照射后4h，结肠隐窝内TUNEL和caspase3阳性细胞减少。与之形成鲜明对比的是，SOD2的表达显著增加。最后，这些研究表明，CeO2纳米颗粒通过(1)作为自由基清除剂和(2)在辐射前增加SOD2的产生来保护胃肠上皮免受辐射损伤。
The ability of rare earth cerium oxide (CeO2) nanoparticles to confer radioprotection against gastrointestinal epithelium was examined. The pretreatment of normal human colon cells (CRL 1541) with varying concentrations of CeO2 nanoparticles 24 hours before single-dose radiation exposure conferred protection from radiation-induced cell death by reducing the amount of reactive oxygen species produced and increasing the expression of superoxide dismutase 2 (SOD2), in a dose-dependent manner. In subsequent experiments athymic nude mice were pretreated with intraperitoneal injections of CeO2 nanoparticles before a single dose of radiation to the abdominal area. Immunohistochemical analysis show a decrease in TUNEL-and caspase 3-positive cells in the colonic crypt, 4 hours after radiation. In sharp contrast, a significant increase in SOD2 expression was observed. In the end, these studies suggest that CeO2 nanoparticles protect the gastrointestinal epithelium against radiation-induced damage by (1) acting as free-radical scavengers and (2) increasing the production of SOD2 before radiation insult.