Functional and structural interactions between measles virus hemagglutinin and CD46
Functional and structural interactions between measles virus hemagglutinin and CD46
复制标题
麻疹病毒血凝素和 CD46 之间的功能和结构相互作用
作者：
Ofer Nussbaum;C. Broder;Bernard Moss;Luli BAR;Shmuel Rozenblatt;Edward A. Berger
我们分析了单个麻疹病毒（MV）表面糖蛋白在介导与人CD 46（主要MV受体）的功能和结构相互作用中的作用。在一个细胞群中，使用重组牛痘病毒载体来产生MV血凝素（H）和融合（F）糖蛋白。作为融合伴侣细胞，检查了各种细胞类型，不含或含有人CD 46（内源性或重组牛痘病毒编码的）。通过定量报告基因激活试验和合胞体形成监测两个细胞群体之间的融合。MV糖蛋白促进与灵长类动物细胞的融合，但不与非灵长类动物细胞;重组CD 46使非灵长类动物细胞有能力进行MV糖蛋白介导的融合。另一种麻疹病毒，犬瘟热病毒（CDV）观察到显着不同的融合特异性：重组CDV糖蛋白促进与灵长类动物和非灵长类动物细胞的融合独立的CD 46。重组MV和CDV糖蛋白的融合需要同源或异源组合的H加F的共表达。为了评估H与F在确定MV融合的CD 46依赖性中的作用，我们检查了产生异源糖蛋白组合的细胞的融合特异性。HMV加FCDV的特异性与观察到的同源MV糖蛋白相同：与灵长类细胞融合，但不与非灵长类细胞融合，除非它们产生重组CD 46。相比之下，HCMV加FMV的特异性超过同源CDV糖蛋白：融合发生在灵长类动物或非灵长类动物细胞中，不依赖于CD 46。因此，对于MV和CDV，融合特异性由H.特别是，结果证明了HMV和CD 46之间的功能性相互作用。流式细胞术和抗体共沉淀研究提供了一个结构相关的功能相互作用：CD 46形成了一个分子复合物与HMV，但不与FMV或CDV糖蛋白。这些结果突出了H糖蛋白在确定MV对CD 46阳性细胞的特异性中的关键作用。
We analyzed the roles of the individual measles virus (MV) surface glycoproteins in mediating functional and structural interactions with human CD46, the primary MV receptor. On one cell population, recombinant vaccinia virus vectors were used to produce the MV hemagglutinin (H) and fusion (F) glycoproteins. As fusion partner cells, various cell types were examined, without or with human CD46 (endogenous or recombinant vaccinia virus encoded). Fusion between the two cell populations was monitored by a quantitative reporter gene activation assay and by syncytium formation. MV glycoproteins promoted fusion with primate cells but not with nonprimate cells; recombinant CD46 rendered nonprimate cells competent for MV glycoprotein-mediated fusion. Markedly different fusion specificity was observed for another morbillivirus, canine distemper virus (CDV): recombinant CDV glycoproteins promoted fusion with primate and nonprimate cells independently of CD46. Fusion by the recombinant MV and CDV glycoproteins required coexpression of H plus F in either homologous or heterologous combinations. To assess the role of H versus F in determining the CD46 dependence of MV fusion, we examined the fusion specificities of cells producing heterologous glycoprotein combinations. The specificity of HMV plus FCDV paralleled that observed for the homologous MV glycoproteins: fusion occurred with primate cells but not with nonprimate cells unless they produced recombinant CD46. By contrast, the specificity of HCDV plus FMV paralleled that for the homologous CDV glycoproteins: fusion occurred with either primate or nonprimate cells with no dependence on CD46. Thus, for both MV and CDV, fusion specificity was determined by H. In particular, the results demonstrate a functional interaction between HMV and CD46. Flow cytometry and antibody coprecipitation studies provided a structural correlate to this functional interaction: CD46 formed a molecular complex with HMV but not with FMV or with either CDV glycoprotein. These results highlight the critical role of the H glycoprotein in determining MV specificity for CD46-positive cells.
影响因子：
3.5
作者：
HO, SN;HUNT, HD;PEASE, LR
通讯作者：
PEASE, LR
影响因子：
3.7
作者：
SERGEL, T;MCGINNES, LW;MORRISON, TG
通讯作者：
MORRISON, TG
CD46（膜辅因子蛋白）的多种亚型可作为麻疹病毒的受体。
DOI：
10.1073/pnas.91.6.2161
发表时间：
1994
影响因子：
11.1
作者：
Manchester,M;Liszewski,MK;Atkinson,JP;Oldstone,MB
通讯作者：
Oldstone,MB