Regulation of bovine satellite cell proliferation and differentiation by insulin and triiodothyronine
Regulation of bovine satellite cell proliferation and differentiation by insulin and triiodothyronine
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DOI：
10.1016/s0739-7240(99)00055-7
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发表时间：
1999-11-01
影响因子：
2.1
通讯作者：
Geay, Y
中科院分区：
文献类型：
--
作者：
Cassar-Malek, I;Langlois, N;Geay, Y
卫星细胞的活动有助于产后肌肉的生长。本研究分别研究了胰岛素和三碘甲状腺原氨酸（T3）对牛半腱肌原代卫星细胞增殖和分化的影响。在基础条件下，卫星细胞增殖至培养第5天，开始融合到肌管中，并表达分化标记物，如连接蛋白、肌原蛋白和肌球蛋白重链（MHC）亚型。胰岛素是一种有效的有丝分裂原。此外，它还促进了广泛的肌管形成，并通过增加分化标记物的积累来增强分化。最大分化发生在胰岛素生理范围浓度。高剂量可促进最大增殖，可延迟刺激分化。相反，T3以剂量依赖的方式降低细胞增殖。此外，融合和生化分化（连接蛋白、MyoD1、肌原素和肌球蛋白重链异构体的积累）也得到增强。牛卫星细胞似乎对胰岛素和T3的增殖有不同的反应。有趣的是，这两种激素都对肌肉产生影响。我们的观察结果表明，这两种激素都可以影响体内牛卫星细胞，并有助于调节产后肌肉生长。(C) 1999 Elsevier Science Inc.；版权所有。
Satellite cells activity contributes to postnatal muscle growth. Herein, we have studied the respective influence of insulin and triiodothyronine (T3) on the proliferation and differentiation of primary bovine satellite cells isolated from Semitendinosus muscle of Montebeliard steers. Under basal conditions, satellite cells proliferated until the fifth day of culture, began to fuse into myotubes and expressed differentiation markers such as connectin, myogenin, and myosin heavy chain (MHC) isoforms. Insulin behaved as an effective mitogen. Moreover, it promoted extensive myotube formation and enhanced differentiation as shown by an increase in the accumulation of differentiation markers. Maximal differentiation occurred with insulin physiological range concentrations. A delay in the stimulation of differentiation was registered with a high dose that promoted maximal proliferation. Conversely, T3 decreased cell proliferation in a dose-dependent manner. In addition, fusion and biochemical differentiation (accumulation of connectin, MyoD1, myogenin, and myosin heavy chain isoforms) were also enhanced. Bovine satellite cells seemed to respond differentially to insulin and T3 for proliferation. Interestingly, both hormones displayed a myogenic influence. Our observations suggest that both hormones could influence bovine satellite cells in vivo and contribute to the regulation of postnatal muscle growth. (C) 1999 Elsevier Science Inc. All rights reserved.