Erythropoietin improves neuronal proliferation in dentate gyrus of hippocampal formation in an animal model of Alzheimer's disease.
Erythropoietin improves neuronal proliferation in dentate gyrus of hippocampal formation in an animal model of Alzheimer's disease.
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DOI：
10.4103/2277-9175.100157
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发表时间：
2012
影响因子：
1
通讯作者：
Reisi P
中科院分区：
文献类型：
--
作者：
Arabpoor Z;Hamidi G;Rashidi B;Shabrang M;Alaei H;Sharifi MR;Salami M;Dolatabadi HR;Reisi P
阿尔茨海默病（Alzheimer's disease，AD）是一种严重的学习和记忆障碍性疾病。由于促红细胞生成素（EPO）已被证明对中枢神经系统有积极作用，本研究的目的是评估EPO对AD明确模型海马结构齿状回神经元增殖的影响。采用双侧侧脑室注射链脲佐菌素（ICV-STZ）的方法建立散发性Alzheimer型痴呆大鼠模型。实验分为对照组、EPO对照组、Alzheimer组和Alzheimer EPO对照组。每隔一天腹腔注射EPO 5000 IU/kg，麻醉后断头取海马齿状回进行免疫组化和Ki 67染色。组织学研究的结果显示，与对照组、对照EPO组和阿尔茨海默病组相比，EPO显著增加了阿尔茨海默病-EPO组海马齿状回中的神经元增殖;然而，其他组之间没有差异。我们的研究结果表明，即使EPO在完整的动物不改变齿状回的神经发生，它仍然可以显着增加神经发生，如果有一个潜在的疾病，如神经退行性疾病。
Alzheimer's disease (AD) is a prevalent disorder with severe learning and memory defects. Because it has been demonstrated that erythropoietin (EPO) has positive effects on the central nervous system, the aim of this study was to evaluate the effect of EPO on neuronal proliferation in dentate gyrus of hippocampal formation in a well-defined model for AD. A rat model of sporadic dementia of Alzheimer's type was established by a bilateral intracerebroventricular injection of streptozotocin (ICV-STZ). Impairment of learning and memory was confirmed 2 weeks after ICV-STZ injection by passive avoidance learning test and then rats were divided into fourgroups:Control, control-EPO, Alzheimer and Alzheimer-EPO. EPO was injected intraperitoneally every other day with a dose of 5000 IU/kg and, finally, the rats were anesthetized and decapitated for immunohistochemical study and neurogenesis investigation (by Ki67 method) in dentate gyrus of hippocampal formation. The results driven from the histological study showed that EPO significantly increases neuronal proliferation in dentate gyrus of hippocampus in the Alzheimer-EPO group compared with the control, control-EPO and Alzheimer groups; however, there were no differences between the other groups. Our results show that even though EPO in intact animals doesnot change neurogenesis in dentate gyrus, it can nonetheless significantly increase neurogenesis if there is an underlying disorder like neurodegenerative diseases.