Corneal confocal microscopy is a rapid reproducible ophthalmic technique for quantifying corneal nerve abnormalities
Corneal confocal microscopy is a rapid reproducible ophthalmic technique for quantifying corneal nerve abnormalities
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DOI：
10.1371/journal.pone.0183040
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发表时间：
2017-08-17
期刊：
影响因子：
3.7
通讯作者：
Malik, Rayaz A.
中科院分区：
文献类型：
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作者：
Kalteniece, Alise;Ferdousi, Maryam;Malik, Rayaz A.
PurposeTo评估应用协议的图像选择的效果和所需的图像的数量进行充分的量化角膜神经病理学使用在体内角膜共焦显微镜（IVCCM）。MethodsIVCCM进行了35名参与者由一个单一的考官。对于每名参与者，4名观察者使用标准化方案选择6张中央角膜神经图像，以评估观察者间的变异性。此外，由一名观察者在两种情况下选择图像，以评估观察者内变异性，并通过比较6张与12张图像来评估样本量的影响。使用全自动软件定量角膜神经纤维密度（CNFD）、分支密度（CNBD）和长度（CNFL）。结果CNFD、CNBD和CNFL的组内相关系数（ICC）分别为0.93（P
PurposeTo assess the effect of applying a protocol for image selection and the number of images required for adequate quantification of corneal nerve pathology using in vivo corneal confocal microscopy (IVCCM).MethodsIVCCM was performed in 35 participants by a single examiner. For each participant, 4 observers used a standardized protocol to select 6 central corneal nerve images to assess the inter-observer variability. Furthermore, images were selected by a single observer on two occasions to assess intra-observer variability and the effect of sample size was assessed by comparing 6 with 12 images. Corneal nerve fiber density (CNFD), branch density (CNBD) and length (CNFL) were quantified using fully automated software. The data were compared using the intra class correlation coefficient (ICC) and Bland-Altman agreement plots for all experiments.ResultsThe ICC values for CNFD, CNBD and CNFL were 0.93 (P