Genetic characterization and evolutionary implications of a chromosomally encoded naphthalene-degradation upper pathway from Pseudomonas stutzeri AN10
Genetic characterization and evolutionary implications of a chromosomally encoded naphthalene-degradation upper pathway from Pseudomonas stutzeri AN10
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DOI：
10.1016/s0378-1119(99)00241-3
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发表时间：
1999-08-05
期刊：
影响因子：
3.5
通讯作者：
Moore, ERB
中科院分区：
文献类型：
--
作者：
Bosch, R;García-Valdés, E;Moore, ERB
施氏假单胞菌AN 10是一株萘降解菌，其异化基因由染色体编码。我们测序了总共11514 bp，包括施氏毕赤酵母AN10的整个萘降解上游途径（nah）。9个开放阅读框架，nahAaAbAcAdBFCED，编码的酶降解萘水杨酸，被确定。斯氏假单胞菌AN10的nah基因已经与编码来自其他假单胞菌萘降解上游途径的同功能蛋白的基因进行了比较。序列同源性的芳香族分解代谢途径的演变的影响进行了讨论。我们的研究结果表明，施氏毕赤酵母AN10的整个分解代谢模块是从其他微生物中招募的，并且在其并入施氏毕赤酵母AN10基因组后的一段短时间内已经过去。比较还表明，两个完整的nah上途径在宿主菌株中共存，并进一步重组。这些事件可能会加速现代分解代谢途径的进化。(C)1999 Elsevier Science B.V.保留所有权利。
Pseudomonas stutzeri strain AN10 is a naphthalene-degrading strain whose dissimilatory genes are chromosomally encoded. We sequenced a total of 11514 bp including the entire naphthalene-degradation upper pathway (nah) of P. stutzeri AN10. Nine open reading frames, nahAaAbAcAdBFCED, encoding the enzymes for the degradation of naphthalene to salicylate, were identified. The nah genes of P. stutzeri AN10 have been compared with genes encoding isofunctional proteins from other Pseudomonas naphthalene-degradation upper pathways. The implications of the sequence homologies to the evolution of aromatic catabolic pathways are discussed. Our findings indicate that this entire catabolic module of P, stutzeri AN10 was recruited from other microorganisms and a short period of time has elapsed after its incorporation within the P. stutzeri AN10 genome. Comparisons also suggest the coexistence of two entire nah upper pathways in a host strain, and further recombination between them. These events could accelerate the evolution of modern catabolic pathways. (C) 1999 Elsevier Science B.V. All rights reserved.